Open Access System for Information Sharing

Login Library

 

Article
Cited 39 time in webofscience Cited 35 time in scopus
Metadata Downloads

MULTIPLE POSITIVE AND NEGATIVE CIS-ACTING ELEMENTS THAT MEDIATE TRANSACTIVATION BY BEL1 IN THE LONG TERMINAL REPEAT OF HUMAN FOAMY VIRUS SCIE SCOPUS

Title
MULTIPLE POSITIVE AND NEGATIVE CIS-ACTING ELEMENTS THAT MEDIATE TRANSACTIVATION BY BEL1 IN THE LONG TERMINAL REPEAT OF HUMAN FOAMY VIRUS
Authors
LEE, AHLEE, KJSUNG, YC
Date Issued
1993-04
Publisher
AMER SOC MICROBIOLOGY
Abstract
The bel1 protein of human foamy virus (HFV), a retrovirus, regulates expression of the gene linked to the HFV long terminal repeat (LTR) and is essential for viral gene expression. The mechanism of action of the bel1 protein is unknown, but its action is mediated through the U3 region of the LTR. To determine which U3 sequences are critical for transactivation by bel1 a series of hybrid vectors consisting of a mutant HFV LTR and the chloramphenicol acetyltransferase gene were constructed and tested for their responsiveness to the bel1 protein by using transient assays after transfection. The target sequences for transactivation by bel1 were mapped to five regions in the U3 domain of the LTR: nucleotides -559 to -506, -454 to -418, -360 to -342, -327 to -284, and -116 to -89 (+1 represents the transcription initiation site). No significant sequence similarity was identified among the five target sites. The observation that the multiple distinct elements in the HFV LTR are the targets for bel1 transactivation is different from observations with other human retroviral systems. The regulation mechanism of HFV bel1 protein-mediated transactivation appears to be analogous to that of some DNA virus transactivators that increase transcription from numerous different viral promoters with little sequence similarity shared among them. We demonstrated that multiple bel1-responsive elements (BRE) can act as bel1-dependent enhancer elements, while a single copy of one BRE, BREe, can serve as an upstream activating element in both orientations. In addition, the region between -466 and -498 was identified as responsible for the downregulation of gene expression directed by BREa, which requires its upstream sequence element to act as a bel1-dependent enhancer element in a heterologous promoter.
URI
https://oasis.postech.ac.kr/handle/2014.oak/11298
DOI
10.1128/JVI.67.4.2317-2326.1993
ISSN
0022-538X
Article Type
Article
Citation
JOURNAL OF VIROLOGY, vol. 67, no. 4, page. 2317 - 2326, 1993-04
Files in This Item:

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

성영철SUNG, YOUNG CHUL
Dept of Life Sciences
Read more

Views & Downloads

Browse