TRANSACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 LONG TERMINAL REPEAT-DIRECTED GENE-EXPRESSION BY THE HUMAN FOAMY VIRUS BEL1 PROTEIN REQUIRES A SPECIFIC DNA-SEQUENCE
SCIE
SCOPUS
- Title
- TRANSACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 LONG TERMINAL REPEAT-DIRECTED GENE-EXPRESSION BY THE HUMAN FOAMY VIRUS BEL1 PROTEIN REQUIRES A SPECIFIC DNA-SEQUENCE
- Authors
- KIM, SY; LEE, AH; LEE, KJ; SUNG, YC
- Date Issued
- 1992-05
- Publisher
- AMER SOC MICROBIOLOGY
- Abstract
- Human foamy virus (HFV) encodes the transcriptional transactivator bell. The bell protein transactivates HFV long terminal repeat (LTR)-directed gene expression by recognizing a region in U3. It also transactivates human immunodeficiency virus type 1 (HIV-1) LTR-directed gene expression in transient transfection assays. To identify the specific region in HIV-1 LTR responsible for bel1 action, we examined the effect of bel1 on chloramphenicol acetyltransferase (CAT) gene expression in transfected cells with a series of mutant HIV-1 LTR/CAT plasmids. The region between -158 and -118 from the transcription initiation site, immediately upstream of the core enhancer element, was identified as responsible for the transactivation by bel1. In addition, bel1 transactivated a heterologous promoter when this region was positioned upstream of it in the sense and antisense orientations. Optimal transactivation of the HIV-1 LTR by bel1 did not require an intact TAR sequence, suggesting that the binding of tat to the TAR sequence is not a prerequisite for bel1 function in HIV-1 LTR-directed gene expression. In the region of the HIV-1 LTR that is necessary for the bel1-mediated transactivation, we have found a sequence which is conserved between HIV-1 and HFV. Our results suggest that the bel1 action on HIV-1 seems to be mediated by a specific DNA sequence which is shared by both the HIV-1 LTR and HFV LTR.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/11297
- DOI
- 10.1128/JVI.66.5.3236-3240.1992
- ISSN
- 0022-538X
- Article Type
- Article
- Citation
- JOURNAL OF VIROLOGY, vol. 66, no. 5, page. 3236 - 3240, 1992-05
- Files in This Item:
-
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.