In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
SCIE
SCOPUS
- Title
- In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
- Authors
- Mai, Thi Thao; Yoo, Su Woong; Park, Suhyun; Kim, Jin Young; Choi, Kang-Ho; Kim, Chulhong; Kwon, Seong Young; Min, Jung-Joon; Lee, Changho
- Date Issued
- 2021-03
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Abstract
- Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/109572
- DOI
- 10.3390/s21051776
- ISSN
- 1424-8220
- Article Type
- Article
- Citation
- Sensors, vol. 21, no. 5, page. 1 - 15, 2021-03
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.