Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
SCIE
SCOPUS
- Title
- Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
- Authors
- Kim, Do-Hyeon; Chang, Yeonho; Park, Soyeon; Jeong, Min Gyu; Kwon, Yonghoon; Zhou, Kai; Noh, Jungeun; Choi, Yun-Kyu; Hong, Triet Minh; Chang, Young-Tae; Ryu, Sung Ho
- Date Issued
- 2021-07
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/107723
- DOI
- 10.1039/d1sc00612f
- ISSN
- 2041-6520
- Article Type
- Article
- Citation
- CHEMICAL SCIENCE, vol. 12, no. 25, 2021-07
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- There are no files associated with this item.
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