Application of Gelatin Bioinks and Cell-Printing Technology to Enhance Cell Delivery Capability for 3D Liver Fibrosis-on-a-Chip Development
SCIE
SCOPUS
- Title
- Application of Gelatin Bioinks and Cell-Printing Technology to Enhance Cell Delivery Capability for 3D Liver Fibrosis-on-a-Chip Development
- Authors
- Lee H.; Kim J.; Choi Y.; Cho D.-W.
- Date Issued
- 2020-04
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Liver fibrosis is a critical liver disease which can lead to liver cirrhosis, cancer, and liver failure. Among various etiological factors, activated stellate cells are a major factor that can induce liver fibrosis. Several studies have presented in vitro models to identify drugs for liver fibrosis; however, there are still limitations in terms of the 2D culture conditions, random co-culture of liver cells, and lack of extracellular matrix components. Therefore, a 3D liver fibrosis-on-a-chip was developed with three liver cell types (hepatocytes, activated stellate cells, and endothelial cells) using a novel cell-printing technique with gelatin bioinks, which were used to deliver each nonparenchymal liver cell type as a multilayer construct. Liver fibrosis-specific gene expression, collagen accumulation, cell apoptosis, and reduced liver functions caused by activated stellate cells were also evaluated. Furthermore, previously reported chemicals were added to the 3D liver fibrosis-on-a-chip to examine the downregulation of activated hepatic stellate cells. In conclusion, the developed 3D liver fibrosis-on-a-chip could be used as a potential in vitro model in the research field.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/107490
- DOI
- 10.1021/acsbiomaterials.9b01735
- ISSN
- 2373-9878
- Article Type
- Article
- Citation
- ACS BIOMATERIALS SCIENCE & ENGINEERING, vol. 6, no. 4, page. 2469 - 2477, 2020-04
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- There are no files associated with this item.
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