Plug-in repressor library for precise regulation of metabolic flux in Escherichia coli
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SCOPUS
- Title
- Plug-in repressor library for precise regulation of metabolic flux in Escherichia coli
- Authors
- Lee, Ji Yeon; Cha, Sanghak; Lee, Ji Hoon; Lim, Hyun Gyu; Noh, Myung Hyun; Kang, Chae Won; Jung, Gyoo Yeol
- Date Issued
- 2021-09
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- In metabolic engineering, enhanced production of value-added chemicals requires precise flux control between growth-essential competing and production pathways. Although advances in synthetic biology have facilitated the exploitation of a number of genetic elements for precise flux control, their use requires expensive inducers, or more importantly, needs complex and time-consuming processes to design and optimize appropriate regulator components, case-by-case. To overcome this issue, we devised the plug-in repressor libraries for target-specific flux control, in which expression levels of the repressors were diversified using degenerate 5' untranslated region (5' UTR) sequences employing the UTR Library Designer. After we validated a wide expression range of the repressor libraries, they were applied to improve the production of lycopene from glucose and 3-hydroxypropionic acid (3-HP) from acetate in Escherichia coli via precise flux re-balancing to enlarge precursor pools. Consequently, we successfully achieved optimal carbon fluxes around the precursor nodes for efficient production. The most optimized strains were observed to produce 2.59 g/L of 3-HP and 11.66 mg/L of lycopene, which were improved 16.5-fold and 2.82-fold, respectively, compared to those produced by the parental strains. These results indicate that carbon flux rebalancing using the plug-in library is a powerful strategy for efficient production of value-added chemicals in E. coli.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/107485
- DOI
- 10.1016/j.ymben.2021.07.013
- ISSN
- 1096-7176
- Article Type
- Article
- Citation
- METABOLIC ENGINEERING, vol. 67, page. 365 - 372, 2021-09
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