Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells
SCIE
SCOPUS
- Title
- Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells
- Authors
- Lee, Minji; Lee, Eunmin; Han, Seong Kyu; Choi, Yoon Ha; Kwon, Dong-il; Choi, Hyobeen; Lee, Kwanghwan; Park, Eun Seo; Rha, Min-Seok; Joo, Dong Jin; Shin, Eui-Cheol; Kim, Sanguk; Kim, Jong Kyoung; LEE, You Jeong
- Date Issued
- 2020-08
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- Invariant natural killer T (iNKT), mucosal-associated invariant T (MAIT), and gamma delta T cells are innate T cells that acquire memory phenotype in the thymus and share similar biological characteristics. However, how their effector differentiation is developmentally regulated is still unclear. Here, we identify analogous effector subsets of these three innate T cell types in the thymus that share transcriptional profiles. Using single-cell RNA sequencing, we show that iNKT, MAIT and gamma delta T cells mature via shared, branched differentiation rather than linear maturation or TCR-mediated instruction. Simultaneous TCR clonotyping analysis reveals that thymic maturation of all three types is accompanied by clonal selection and expansion. Analyses of mice deficient of TBET, GATA3 or ROR gamma t and additional in vivo experiments corroborate the predicted differentiation paths, while human innate T cells from liver samples display similar features. Collectively, our data indicate that innate T cells share effector differentiation processes in the thymus. Innate T cells such as iNKT, MAIT and gamma delta T cells all develop in the thymus, but their differentiation paths are still unclear. Here, the authors show, using single-cell RNA sequencing, that all three cell types develop via shared and branched differentiation paths that are corroborated by additional results from gene-deficient mice and human liver T cells.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/107152
- DOI
- 10.1038/s41467-020-18155-8
- ISSN
- 2041-1723
- Article Type
- Article
- Citation
- NATURE COMMUNICATIONS, vol. 11, no. 1, 2020-08
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- There are no files associated with this item.
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