Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics
SCIE
SCOPUS
- Title
- Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics
- Authors
- LIM, GEUN BAE; Jongyoon Han; Hyunryul Ryu; Bruce D. Levy; Kyungyong Choi; Bakr Jundi; JEON, HYUNG KOOK
- Date Issued
- 2020-10
- Publisher
- ROYAL SOC CHEMISTRY
- Abstract
- A fully-automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, similar to 80% WBC recovery, >85% WBC purity, and similar to 12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 mu L of human peripheral blood (50 mu L), without any cell damage or phenotypic changes in a fully-automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/107144
- DOI
- 10.1039/d0lc00675k
- ISSN
- 1473-0197
- Article Type
- Article
- Citation
- LAB ON A CHIP, vol. 20, no. 19, page. 3612 - 3624, 2020-10
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.