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Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins SCIE SCOPUS

Title
Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins
Authors
Choi, DongsicGo, GyeongyunKim, Dae-KyumLee, JaewookPark, Seon-MinDi Vizio, DoloresGho, Yong Song
Date Issued
2020-01
Publisher
TAYLOR & FRANCIS LTD
Abstract
Extracellular vesicles (EVs) are nano-sized vesicles surrounded by a lipid bilayer and released into the extracellular milieu by most of cells. Although various EV isolation methods have been established, most of the current methods isolate EVs with contaminated non-vesicular proteins. By applying the label-free quantitative proteomic analyses of human colon cancer cell SW480-derived EVs, we identified trypsin-sensitive and trypsin-resistant vesicular proteins. Further systems biology and protein-protein interaction network analyses based on their cellular localization, we classified the trypsin-sensitive and trypsin-resistant vesicular proteins into two subgroups: 363 candidate real-vesicular proteins and 151 contaminated non-vesicular proteins. Moreover, the protein interaction network analyses showed that candidate real-vesicular proteins are mainly derived from plasma membrane (46.8%), cytosol (36.6%), cytoskeleton (8.0%) and extracellular region (2.5%). On the other hand, most of the contaminated non-vesicular proteins derived from nucleus, Golgi apparatus, endoplasmic reticulum and mitochondria. In addition, ribosomal protein complexes and T-complex proteins were classified as the contaminated non-vesicular proteins. Taken together, our trypsin-digested proteomic approach on EVs is an important advance to identify the real-vesicular proteins that could help to understand EV biogenesis and protein cargo-sorting mechanism during EV release, to identify more reliable EV diagnostic marker proteins, and to decode pathophysiological roles of EVs.
URI
https://oasis.postech.ac.kr/handle/2014.oak/106964
DOI
10.1080/20013078.2020.1757209
ISSN
2001-3078
Article Type
Article
Citation
JOURNAL OF EXTRACELLULAR VESICLES, vol. 9, no. 1, 2020-01
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고용송GHO, YONG SONG
Dept of Life Sciences
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