Environment-insensitive two-photon ratiometric probe for in cellulo quantitative measurement of hydrogen peroxide
SCOPUS
- Title
- Environment-insensitive two-photon ratiometric probe for in cellulo quantitative measurement of hydrogen peroxide
- Authors
- Cho, S.W.; Jun, Y.W.; Reo, Y.J.; Sarkar, S.; Ahn, K.H.
- Date Issued
- 2021-01
- Publisher
- Elsevier B.V.
- Abstract
- The quantitative analysis of a biological analyte directly through fluorescence imaging is essential in biomedical sciences but remains as a challenge owing to the environment-sensitive nature of fluorescence intensity. We show that a fluorescent hydrogen peroxide probe based on an in cellulo super-bright benzothiazolyl-benzocoumarin dye of which emission properties are insensitive to environmental changes offers a reliable and practical means for the quantitative analysis of hydrogen peroxide in cells and tissues directly through ratiometric imaging both under one-photon and two-photon excitation conditions. Different cellular hydrogen peroxide concentrations were determined for several cell lines and also for tumour and normal tissues. © 2021 The Author(s)
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/106886
- DOI
- 10.1016/j.rechem.2021.100117
- ISSN
- 2211-7156
- Article Type
- Article
- Citation
- Results in Chemistry, vol. 3, 2021-01
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- There are no files associated with this item.
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