Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production
SCIE
SCOPUS
- Title
- Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production
- Authors
- Han, Mee-Jung; Lee, Jeong Wook; Lee, Sang Yup; Yoo, Jong Shin
- Date Issued
- 2008-01
- Publisher
- Hindawi Publishing Corporation
- Abstract
- InEscherichia coli, a long-chain acyl-CoA is a regulatory signal that modulates gene expression through its binding to a transcription factor FadR. In this study, comparative proteomic analysis ofE. coliin the presence of glucose and oleic acid was performed to understand cell physiology in response to oleic acid. Among total of 52 proteins showing altered expression levels with oleic acid presence, 9 proteins including AldA, Cdd, FadA, FadB, FadL, MalE, RbsB, Udp, and YccU were newly synthesized. Among the genes that were induced by oleic acid, the promoter of thealdAgene was used for the production of a green fluorescent protein (GFP). Analysis of fluorescence intensities and confocal microscopic images revealed that soluble GFP was highly expressed under the control of thealdApromoter. These results suggest that proteomics is playing an important role not only in biological research but also in various biotechnological applications.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/105772
- DOI
- 10.1155/2008/735101
- ISSN
- 1110-7243
- Article Type
- Article
- Citation
- Journal of Biomedicine and Biotechnology, vol. 2008, page. 1 - 12, 2008-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.