Development of a new extrusion-based 3D printing method and its application to esophagus tissue engineering
- Title
- Development of a new extrusion-based 3D printing method and its application to esophagus tissue engineering
- Authors
- Jeong, Hun-Jin; Nam, Hyoryung; Jo, Yeonggwon; Lee, Jae Yeon; Ha, Dong-Heon; Kim, Ji Hyun; Chung, Jae Hee; Cho, Young-Sam; Cho, Dong-Woo; Jang, Jinah; Lee, Seung-Jae
- Date Issued
- 2020-11-16
- Publisher
- 한국정밀공학회
- Abstract
- Most of the tubular tissues and organs in the human body have a hierarchical structure with various cellular
components. For this, in the field of tubular tissue engineering have been widely studying to fabricate of the multilayered construct using the various manufacturing technique such as electrospinning, cell-sheet, 3D bioprinting.
Despite many attempts of the tissue engineered approaches, there are still challenges to create the functional 3D
structure which has a biomimetic multi-layered construct and multi-cellular component similar to the native tissue.
In this study, we developed an extrusion-based 3D printing method (dragging technique) which can build the
multi-layered free-form 3D tubular structure with the controllable pore structure on each layer. Additionally, the
dragging technique can allow the embedding of the various biophysical and biochemical materials, including
target cells into the 3D printed multi-layered construct. Based on the suggested technique, we fabricated a
bioprinted 3D esophageal structure with biomimetic morphological features and converged with biochemical
microenvironmental cue of an esophageal tissue by using porcine derived decellularized bioinks from mucosa and
muscular tissue. The fabricated the esophagus construct using the dragging technique has not only to produce a
controllable pore structure, free-form, multi-layered but also mimic the multi-cellular arrangement of the actual
esophagus. The esophagus constructs investigated as suitable for alternative esophagus regeneration through the
in-vitro experiments.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/105646
- Article Type
- Conference
- Citation
- PRESM 2020, page. 67 - 67, 2020-11-16
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