Open Access System for Information Sharing

Login Library

 

Article
Cited 9 time in webofscience Cited 10 time in scopus
Metadata Downloads

Aqueous Two-Phase System (ATPS)-Based Polymersomes for Particle Isolation and Separation SCIE SCOPUS

Title
Aqueous Two-Phase System (ATPS)-Based Polymersomes for Particle Isolation and Separation
Authors
Seo, HanjinNam, ChangwooKim, EunseoSon, JuhyunLee, Hyomin
Date Issued
2020-12
Publisher
ACS
Abstract
Aqueous two-phase systems (ATPSs) have been widely used in the separation, purification, and enrichment of biomolecules for their excellent biocompatibility. While ultra-centrifugation and microfluidic devices have been combined with ATPS to facilitate the separation of biomolecules and achieve high recovery yields, they often lack the ability to effectively isolate and separate biomolecules in low concentrations. In this work, we present a strategy that leverages the preferential partitioning of biomolecules in ATPS droplets to efficiently separate model extracellular vesicle (EV) particles. We demonstrate that the additional oil phase between the inner ATPS droplets and the aqueous continuous phase in triple emulsion droplets resolves the size controllability and instability issues of ATPS droplets, enabling the production of highly monodisperse ATPS-based polymersomes with enhanced stability for effective isolation of ATPS droplets from the surrounding environment. Furthermore, we achieve separation of model EV particles in a single dextran (DEX)-rich droplet by the massive production of ATPS-based polymersomes and osmotic-pressure-induced rupture of the selected polymersome in a hypertonic solution composed of poly(ethylene glycol) (PEG).
URI
https://oasis.postech.ac.kr/handle/2014.oak/104722
DOI
10.1021/acsami.0c16968
ISSN
1944-8244
Article Type
Article
Citation
ACS Applied Materials and Interfaces, vol. 12, no. 49, page. 55467 - 55475, 2020-12
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

이효민Lee, Hyomin
Dept. of Chemical Enginrg
Read more

Views & Downloads

Browse