Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103(+)CD11b(+) Dendritic Cells
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SCOPUS
- Title
- Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103(+)CD11b(+) Dendritic Cells
- Authors
- Ko, Hyun-Ja; Hong, Sung-Wook; Verma, Ravi; Jung, Jisun; Lee, Minji; Kim, Nahyun; Kim, Daeun; Surh, Charles D.; Kim, Kwang Soon; Rudra, Dipayan; IM, SIN HYEOG
- Date Issued
- 2020-08
- Publisher
- FRONTIERS MEDIA SA
- Abstract
- Retinal dehydrogenase (RALDH) enzymatic activities catalyze the conversion of
vitamin A to its metabolite Retinoic acid (RA) in intestinal dendritic cells (DCs)
and promote immunological tolerance. However, precise understanding of the exogenous
factors that act as initial trigger of RALDH activity in these cells is still
evolving. By using germ-free (GF) mice raised on an antigen free (AF) elemental
diet, we find that certain components in diet are critically required to establish
optimal RALDH expression and activity, most prominently in small intestinal
CD103(+)CD11b(+) DCs (siLP-DCs) right from the beginning of their lives.
Surprisingly, systematic screens using modified diets devoid of individual dietary
components indicate that proteins, starch and minerals are dispensable for this
activity. On the other hand, in depth comparison between subtle differences in
dietary composition among different dietary regimes reveal that adequate glucose
concentration in diet is a critical determinant for establishing RALDH activity
specifically in siLP-DCs. Consequently, pre-treatment of siLP-DCs, and not
mesenteric lymph node derived MLNDCs with glucose, results in significant
enhancement in the in vitro generation of induced Regulatory T (iTreg) cells. Our
findings reveal previously underappreciated role of dietary glucose concentration in
establishing regulatory properties in intestinal DCs, thereby extending a potential
therapeutic module against intestinal inflammation
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/104223
- DOI
- 10.3389/fimmu.2020.01897
- ISSN
- 1664-3224
- Article Type
- Article
- Citation
- FRONTIERS IN IMMUNOLOGY, vol. 11, page. 1897, 2020-08
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