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CrABCA2 Facilitates Triacylglycerol Accumulation in Chlamydomonas reinhardtii under Nitrogen Starvation

Title
CrABCA2 Facilitates Triacylglycerol Accumulation in Chlamydomonas reinhardtii under Nitrogen Starvation
Authors
Youngsook LeeSUNGHOON, JANGFantao KongJihyeon LeeBae Young ChoiPengfei WangPeng GaoTakashi YamanoHideya FukuzawaByung-Ho Kang
Date Issued
Jan-2020
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Abstract
The microalga Chlamydomonas reinhardtii accumulates triacylglycerols ( TAGs) in lipid droplets under stress conditions, such as nitrogen starvation. TAG biosynthesis occurs mainly at the endoplasmic reticulum (ER) and requires fatty acid (FA) substrates supplied from chloroplasts. How FAs are transferred from chloroplast to ER in microalgae was unknown. We previously reported that an Arabidopsis thaliana ATP-binding cassette (ABC) transporter, AtABCA9, facilitates FA transport at the ER during seed development. Here we identified a gene homologous to AtABCA9 in the C. reinhardtii genome, which we named CrABCA2. Under nitrogen deprivation conditions, CrABCA2 expression was upregulated, and the CrABCA2 protein level also increased. CrABCA2 knockdown lines accumulated less TAGs and CrABCA2 overexpression lines accumulated more TAGs than their untransformed parental lines. Transmission electron microscopy showed that CrABCA2 was localized in swollen ER. These results suggest that CrABCA2 transports substrates for TAG biosynthesis to the ER during nitrogen starvation. Our study provides a potential tool for increasing lipid production in microalgae.
Keywords
ABC TRANSPORTERS; LIPID-METABOLISM; IDENTIFICATION; ACYLTRANSFERASES; GENETICS; PROTEIN; REGULATOR; EVOLUTION; ALGAE
URI
http://oasis.postech.ac.kr/handle/2014.oak/101140
ISSN
1016-8478
Article Type
Article
Citation
MOLECULES AND CELLS, vol. 43, no. 1, page. 48 - 57, 2020-01
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이영숙LEE, YOUNGSOOK
Dept of Life Sciences
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