Bio-orthogonal Supramolecular Latching inside Live Animals and Its Application for in Vivo Cancer Imaging
SCIE
SCOPUS
- Title
- Bio-orthogonal Supramolecular Latching inside Live Animals and Its Application for in Vivo Cancer Imaging
- Authors
- Li Meng; SUNGWAN, KIM; ARA, LEE; Shrinidhi Annadka; Young Ho, Ko; LIM, HAEGYUN; KIM, HYUNG HAM; Park, Kyeung Min; KIM, KIMOON
- Date Issued
- 2019-11
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Here, we demonstrate a supramolecular latching tool for bio-orthogonal noncovalent anchoring of small synthetic molecules in live animal models using a fully synthetic high-affinity binding pair between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). This supramolecular latching system is small (similar to 1 kDa), ensuring efficient uptake into cells, tissues, and whole organisms. It is also chemically robust and resistant to enzymatic degradation and analogous to well-characterized biological systems in terms of noncovalent binding. Occurrence of fluorescence resonance energy transfer (FRET) between cyanine 3-CB[7] (Cy3-CB[7]) and boron-dipyrromethene 630/650X-AdA (BDP630/650-AdA) inside a live worm (Caenorhabditis elegans) indicates efficient in situ high-affinity association between AdA and CB[7] inside live animals. In addition, selective visualization of a cancer site of a live mouse upon supramolecular latching of cyanine 5-AdA (Cy5-AdA) on prelocalized CB[7]-conjugating antibody on the cancer site demonstrates the potential of this synthetic system for in vivo cancer imaging. These findings provide a fresh insight into the development of new chemical biology tools and medical therapeutic systems.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/100435
- DOI
- 10.1021/acsami.9b16283
- ISSN
- 1944-8244
- Article Type
- Article
- Citation
- ACS APPLIED MATERIALS & INTERFACES, vol. 11, no. 47, page. 43920 - 43927, 2019-11
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