Endosomal signalling via exosome surface TGF beta-1
SCIE
SCOPUS
- Title
- Endosomal signalling via exosome surface TGF beta-1
- Authors
- Shelke, Ganesh Vilas; Yin, Yanan; Jang, Su Chul; Lasser, Cecilia; Wennmalm, Stefan; Hoffmann, Hans Jurgen; Li, Li; Gho, Yong Song; Nilsson, Jonas Andreas; Lotvall, Jan
- Date Issued
- 2019-12
- Publisher
- Co-Action Publishing
- Abstract
- Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor beta-1 (TGF beta-1) on their surfaces. The latent form of TGF beta-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGF beta-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGF beta-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGF beta-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/100381
- DOI
- 10.1080/20013078.2019.1650458
- ISSN
- 2001-3078
- Article Type
- Article
- Citation
- Journal of Extracellular Vesicles, vol. 8, no. 1, 2019-12
- Files in This Item:
-
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.