A Rapid Colorimetric Sensor for Soluble Interleukin-2 Receptor alpha, Based on Aptamer-Adsorbed AuNP
SCIE
SCOPUS
- Title
- A Rapid Colorimetric Sensor for Soluble Interleukin-2 Receptor alpha, Based on Aptamer-Adsorbed AuNP
- Authors
- Jeon, J.; Jo, H.; Her, J.; Youn, H.; Park, J.; Jo, J.; Lee, J.; Chang, C.L.; Ban, C.
- Date Issued
- 2019-09
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- The soluble interleukin-2 receptor alpha (sIL-2R alpha) is a broad indicator of clinical disease activity in various inflammatory diseases. Here we have developed, for the first time, a rapid, washing-free colorimetric aptasensor based on a sIL-2R alpha aptamer (K-d=1.33 nm). The aptasensor was fabricated with Au nanoparticles (AuNPs) adsorbing sIL-2R alpha aptamers. On addition of sIL-2R alpha, the aptamers become desorbed from the AuNPs, and this in turn weakens the absorption corresponding to AuNP-catalyzed oxidation of ortho-phenylenediamine (oPD) with H2O2. The aptasensor was characterized by TEM imaging, zeta potential measurements, dynamic light scattering (DLS) analysis, and UV/Vis spectrometry, followed by further optimization. The fabricated sensor exhibited great analytical performance, with a linear range of 1 to 100 nm and a detection limit of 1 nm both in buffer and in spiked human serum within 25 min. Other proteins, such as bovine serum albumin (BSA), IL-17R alpha, IL-5R alpha, IL-13R alpha(2), and CD166, showed negligible effects on the aptasensor. Thanks to the great advantages of the aptamers and AuNPs, this aptasensor provides a rapid, simple, and inexpensive process that might offer insights into various diagnostic applications of sIL-2R alpha.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/100304
- DOI
- 10.1002/cbic.201900109
- ISSN
- 1439-4227
- Article Type
- Article
- Citation
- CHEMBIOCHEM, vol. 20, no. 17, page. 2236 - 2240, 2019-09
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