DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, ST | - |
dc.contributor.author | Kerjan, G | - |
dc.contributor.author | Bielas, SL | - |
dc.contributor.author | Lee, JE | - |
dc.contributor.author | Fenstermaker, AG | - |
dc.contributor.author | Novarino, G | - |
dc.contributor.author | Gleeson, JG | - |
dc.date.accessioned | 2017-07-19T13:29:35Z | - |
dc.date.available | 2017-07-19T13:29:35Z | - |
dc.date.created | 2017-02-07 | - |
dc.date.issued | 2014-06-18 | - |
dc.identifier.issn | 0896-6273 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/37088 | - |
dc.description.abstract | Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing brain is a powerful technique to study genetic function; however, discrepancies between knockdown and knockout murine phenotypes have left unanswered questions. For example, doublecortin (Dcx) knockdown but not knockout shows a neocortical neuronal migration phenotype. Here we report that in utero electroporation of shRNA, but not siRNA or miRNA, to Dcx demonstrates a migration phenotype in Dcx knockouts akin to the effect in wild-type mice, suggesting shRNA-mediated off-target toxicity. This effect was not limited to Dcx, as it was observed in Dclk1 knockouts, as well as with a fraction of scrambled shRNAs, suggesting a sequence-dependent but not sequence-specific effect. Profiling RNAs from electroporated cells showed a defect in endogenous let7 miRNA levels, and disruption of let7 or Dicer recapitulated the migration defect. The results suggest that shRNA-mediated knockdown can produce untoward migration effects by altering endogenous miRNA pathways. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.relation.isPartOf | NEURON | - |
dc.title | Off-Target Effect of doublecortin Family shRNA on Neuronal Migration Associated with Endogenous MicroRNA Dysregulation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/J.NEURON.2014.04.036 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | NEURON, v.82, no.6, pp.1255 - 1262 | - |
dc.identifier.wosid | 000337360700012 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1262 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1255 | - |
dc.citation.title | NEURON | - |
dc.citation.volume | 82 | - |
dc.contributor.affiliatedAuthor | Baek, ST | - |
dc.identifier.scopusid | 2-s2.0-84902438430 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 50 | - |
dc.description.scptc | 51 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | RADIAL MIGRATION | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | NEUROGENESIS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INTERFERENCE | - |
dc.subject.keywordPlus | NEOCORTEX | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.