DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung Hee Jung | - |
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | De Wei, Q | - |
dc.contributor.author | CHA, HYUNG JOON | - |
dc.date.accessioned | 2017-07-18T16:41:44Z | - |
dc.date.available | 2017-07-18T16:41:44Z | - |
dc.date.created | 2010-11-04 | - |
dc.date.issued | 2008-01 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/30958 | - |
dc.description.abstract | The effective delivery of exogenous genes into eukaryotic cells is important for fundamental and biotechnological research. Protein-based gene delivery including histone proteins has recently emerged as a powerful technique for non-viral DNA transfer. Histories are DNA-binding proteins that function in DNA packaging and protection. In particular, histone H1 is largely responsible for the stabilization of higher-order chromatin structures. Several studies have examined the use of full-length histone H1-mediated gene transfer, and a few studies have investigated the use of C-terminal histone H1 fragments as gene-transfer materials. Previously, we cloned a novel histone H1 cDNA from the goldfish Carassius auratus and found that a recombinant histone H1 C-terminal short peptide (H1C) of 61 amino acids has comparable DNA binding and protection functions as full-length histone H1. In the present work, we successfully expressed and purified soluble recombinant H1 C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons. We confirmed its DNA-binding ability and found that this H1C peptide had similar or higher transfection efficiency in mammalian cells (human 293T and mouse NIH/3T3) than the widely used agent lipofectamine. Therefore, we suggest that this novel goldfish-derived recombinant histone H1 C-terminal short peptide could be used as a peptide-based gene-transfer mediator. | - |
dc.language | English | - |
dc.publisher | Wiley | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.title | Carassius auratus-originated recombinant histone H1 c-terminal peptide as Gene delivery material | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/BP070069B | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, v.24, no.1, pp.17 - 22 | - |
dc.identifier.wosid | 000252878700004 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.endPage | 22 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 17 | - |
dc.citation.title | BIOTECHNOLOGY PROGRESS | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | CHA, HYUNG JOON | - |
dc.identifier.scopusid | 2-s2.0-38949155209 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 5 | - |
dc.description.scptc | 5 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article; Proceedings Paper | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | H1-MEDIATED TRANSFECTION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | H1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
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