Crystal structure of the human GINS complex

Abstract

The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex. The eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which must be assembled for cell proliferation. We determined the crystal structure of the human GINS complex: GINS forms an elliptical shape with a small central channel. The structures of Sld5 and Psf2 resemble those of Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal domains of Sld5/ Psf1 are permuted in Psf2/ Psf3, which suggests that the four proteins have evolved from a common ancestor. Using a structure- based mutational analysis, we identified the functionally critical surface regions of the GINS complex.