DC Field | Value | Language |
---|---|---|
dc.contributor.author | SEO, JONGCHEOL | - |
dc.contributor.author | Suh, MS | - |
dc.contributor.author | Thangadurai, TD | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Rhee, YH | - |
dc.contributor.author | Yoon, HJ | - |
dc.contributor.author | Shin, SK | - |
dc.date.accessioned | 2016-04-01T09:04:52Z | - |
dc.date.available | 2016-04-01T09:04:52Z | - |
dc.date.created | 2009-03-05 | - |
dc.date.issued | 2008-08-15 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.other | 2008-OAK-0000010907 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29414 | - |
dc.description.abstract | Mass-balanced H-1/H-2 isotope dipeptide tags (MBITs) are presented for simultaneous protein quantitation and identification. MBIT is derived from N-acetyl-Ala-Ala dipeptide and conjugated to primary amines of target peptides. H-1/H-2 isotopes are encoded in the methyl groups of N-acetylated dipeptide: one tag deuterated on the N-acetyl group and another on the C-terminal alanine. MBIT-linked peptides comigrate in reversed-phase liquid chromatography without significant H-1/H-2 isotope effects and provide 2-plex quantitation signals at 114 and 117 Th as well as peptide sequence information upon MS/MS analysis with MALDI TOF/TOF. MBIT shows good quantitation linearity in a concentration range of 20-250 fmol. The performance of MBIT on protein quantitation and identification is further tested with yeast heat-shock protein (Hsp82p) obtained from three different physiological states. MBIT using nanogram-scale samples produces the relative abundance ratios comparable to those obtained from optical imaging of microgram-scale samples visualized with SYPRO Ruby stain. The MBIT strategy is a simple and low-cost alternative for 2-plex quantitation of proteins and offers possibilities of tuning the 2-plex signal mass window by replacing the N-terminal alanine with other amino acid residues. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.title | Mass-balanced H-1/H-2 isotope dipeptide tag for simultaneous protein quantitation and identification | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1021/AC801007Y | - |
dc.author.google | Seo, J | - |
dc.author.google | Suh, MS | - |
dc.author.google | Thangadurai, TD | - |
dc.author.google | Kim, J | - |
dc.author.google | Rhee, YH | - |
dc.author.google | Yoon, HJ | - |
dc.author.google | Shin, SK | - |
dc.relation.volume | 80 | - |
dc.relation.issue | 16 | - |
dc.relation.startpage | 6145 | - |
dc.relation.lastpage | 6153 | - |
dc.contributor.id | 10153778 | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.80, no.16, pp.6145 - 6153 | - |
dc.identifier.wosid | 000258448100001 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 6153 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 6145 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 80 | - |
dc.contributor.affiliatedAuthor | SEO, JONGCHEOL | - |
dc.contributor.affiliatedAuthor | Rhee, YH | - |
dc.contributor.affiliatedAuthor | Shin, SK | - |
dc.identifier.scopusid | 2-s2.0-50049105086 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.description.scptc | 10 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | 2-DIMENSIONAL GEL-ELECTROPHORESIS | - |
dc.subject.keywordPlus | CODED AFFINITY TAGS | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | SPECTROMETRY | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | STRATEGY | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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