DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang, CW | - |
dc.contributor.author | Choi, DH | - |
dc.contributor.author | Kim, WJ | - |
dc.contributor.author | Yockman, JW | - |
dc.contributor.author | Christensen, LV | - |
dc.contributor.author | Kim, YH | - |
dc.contributor.author | Kim, SW | - |
dc.date.accessioned | 2016-04-01T08:50:57Z | - |
dc.date.available | 2016-04-01T08:50:57Z | - |
dc.date.created | 2009-09-30 | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.other | 2007-OAK-0000016689 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28903 | - |
dc.description.abstract | Naked plasmid DNA (pDNA)-based gene therapy has low delivery efficiency, and consequently, low therapeutic effect. We present a biodegradable nonionic triblock copolymer, PEG(13)-PLGA(10)-PEG(13), to enhance gene delivery efficiency in skeletal muscle. Effects of PEG(13)PLGA(10)-PEG(13) on physicochemical properties of pDNA were evaluated by atomic force microscopy (AFM) imaging, gel electrophoresis and zeta-potential analysis. AFM imaging suggested a slightly compacted structure of pDNA when it was mixed with the polymer, while zeta-potential measurement indicated an increased surface potential of negatively charged pDNA. PEG(13)-PLGA(10)-PEG(13) showed a relatively lower toxicity compared to Pluronic P85 in a skeletal muscle cell line. The luciferase expression of pDNA delivered in 0.25% polymer solution was up to three orders of magnitude more than branched polyethylenimine (bPEI(25 k))/pDNA and three times more than that of naked pDNA five days after intramuscular administration. This in vivo gene delivery enhancement was also observed displaying a two-fold higher expression of human vascular endothelial growth factor (VEGF). Based on fluorescence labeled pDNA distribution, it is speculated that the greater diffusivity of PEG(13)-PLGA(10)-PEG(13)/pDNA compared to bPET(25 k)/pDNA accounts for better transfection efficiency in vivo. To summarize, combining PEG(13)-PLGA(10)-PEG(13) with pDNA possesses the potential to improve gene delivery efficiency in skeletal muscle. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.title | Non-ionic amphiphilic biodegradable PEG-PLGA-PEG copolymer enhances gene delivery efficiency in rat skeletal muscle | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1016/j.jconrel.2006.11.025 | - |
dc.author.google | Chang, CW | - |
dc.author.google | Choi, DH | - |
dc.author.google | Christensen, LV | - |
dc.author.google | Kim, SW | - |
dc.author.google | Kim, WJ | - |
dc.author.google | Kim, YH | - |
dc.author.google | Yockman, JW | - |
dc.relation.volume | 118 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 245 | - |
dc.relation.lastpage | 253 | - |
dc.contributor.id | 10135304 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.118, no.2, pp.245 - 253 | - |
dc.identifier.wosid | 000245498500012 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 253 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 245 | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 118 | - |
dc.contributor.affiliatedAuthor | Kim, WJ | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 41 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | WATER-SOLUBLE LIPOPOLYMER | - |
dc.subject.keywordPlus | GROWTH-FACTOR GENE | - |
dc.subject.keywordPlus | BLOCK-COPOLYMERS | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | THERAPEUTIC ANGIOGENESIS | - |
dc.subject.keywordPlus | INTRAMUSCULAR INJECTION | - |
dc.subject.keywordPlus | LIMB ISCHEMIA | - |
dc.subject.keywordPlus | MOUSE MUSCLE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | gene therapy | - |
dc.subject.keywordAuthor | PEG-PLGA-PEG | - |
dc.subject.keywordAuthor | skeletal muscle | - |
dc.subject.keywordAuthor | plasmid DNA | - |
dc.subject.keywordAuthor | VEGF | - |
dc.subject.keywordAuthor | biodegradable | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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