DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, EJ | - |
dc.contributor.author | Park, K | - |
dc.contributor.author | Choi, JS | - |
dc.contributor.author | Joo, CK | - |
dc.contributor.author | Hahn, SK | - |
dc.date.accessioned | 2016-04-01T08:22:14Z | - |
dc.date.available | 2016-04-01T08:22:14Z | - |
dc.date.created | 2009-11-20 | - |
dc.date.issued | 2009-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.other | 2009-OAK-0000019325 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/27820 | - |
dc.description.abstract | Anti-Flt1 peptide of GNQWFI has been reported to inhibit vascular endothelial growth factor receptor 1 (VEGFR1) - mediated endothelial cell migration and tube formation. In this work, a protocol to synthesize anti-Flt1 peptide-hyaluronate (HA) conjugate was successfully developed for the treatment of corneal neovascularization. Using tetrabutyl ammonium salt of HA (HA-TBA), water-insoluble anti-Flt1 peptide could be conjugated with HA in dimethyl sulfoxide (DMSO) by the amide bond formation between carboxyl groups of HA and N-terminal amine groups of GGNQWFI. The formation of anti-Flt1 peptide-HA conjugate was confirmed by H-1 NMR and fluorometric analyses. The average number of grafted peptide molecules in anti-Flt1 peptide-HA conjugates could be controlled from 3 to 30 per single HA chain by changing the feeding amount of peptide for the conjugation reaction. According to in vitro biological activity tests, anti-Flt1 peptide-HA conjugate exhibited a significant inhibition effect on the binding of Flt1-Fc to VEGF(165) coated on the well. Furthermore, in vivo biological activity of anti-Flt1 peptide-HA conjugate was confirmed from the inhibitory effect on corneal neovascularization in silver nitrate cauterized corneas of SD rats. The VEGF receptor 2 expression was also reduced after treatment with anti-Flt1 peptide-HA conjugate. The water-soluble anti-Flt1 peptide-HA conjugate was thought to have a potential to be developed as anti-angiogenic therapeutics for the treatment of corneal neovascularization. (C) 2009 Elsevier Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.subject | Hyaluronate | - |
dc.subject | Anti-Flt1 peptide | - |
dc.subject | Conjugation | - |
dc.subject | Drug delivery | - |
dc.subject | Corneal neovascularization | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | SUSTAINED-RELEASE FORMULATION | - |
dc.subject | ANGIOGENIC OLIGOSACCHARIDES | - |
dc.subject | SODIUM HYALURONATE | - |
dc.subject | SIGNALING PATHWAYS | - |
dc.subject | TYROSINE KINASE | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | TUMOR-GROWTH | - |
dc.subject | RECEPTOR | - |
dc.subject | ACID | - |
dc.title | Synthesis, characterization, and preliminary assessment of anti-Flt1 peptide-hyaluronate conjugate for the treatment of corneal neovascularization | - |
dc.type | Article | - |
dc.contributor.college | 신소재공학과 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2009.07.024 | - |
dc.author.google | Oh, EJ | - |
dc.author.google | Park, K | - |
dc.author.google | Choi, JS | - |
dc.author.google | Joo, CK | - |
dc.author.google | Hahn, SK | - |
dc.relation.volume | 30 | - |
dc.relation.issue | 30 | - |
dc.relation.startpage | 6026 | - |
dc.relation.lastpage | 6034 | - |
dc.contributor.id | 10149037 | - |
dc.relation.journal | BIOMATERIALS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.30, no.30, pp.6026 - 6034 | - |
dc.identifier.wosid | 000270204500015 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 6034 | - |
dc.citation.number | 30 | - |
dc.citation.startPage | 6026 | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 30 | - |
dc.contributor.affiliatedAuthor | Hahn, SK | - |
dc.identifier.scopusid | 2-s2.0-69249212196 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 33 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | SUSTAINED-RELEASE FORMULATION | - |
dc.subject.keywordPlus | ANGIOGENIC OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | SODIUM HYALURONATE | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | TYROSINE KINASE | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | TUMOR-GROWTH | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | Hyaluronate | - |
dc.subject.keywordAuthor | Anti-Flt1 peptide | - |
dc.subject.keywordAuthor | Conjugation | - |
dc.subject.keywordAuthor | Drug delivery | - |
dc.subject.keywordAuthor | Corneal neovascularization | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
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