DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, L | - |
dc.contributor.author | Kim young soo | - |
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Seo Junh hyun | - |
dc.contributor.author | Jung Hee Jung | - |
dc.contributor.author | Wuan Du | - |
dc.contributor.author | Cha Hyung Joon | - |
dc.date.accessioned | 2016-04-01T02:44:54Z | - |
dc.date.available | 2016-04-01T02:44:54Z | - |
dc.date.created | 2010-11-04 | - |
dc.date.issued | 2007-04-15 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.other | 2007-OAK-0000021801 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25695 | - |
dc.description.abstract | Previously, we found that baculoviral polyhedrin (Polh) can successfully be used in Escherichia coli as a fusion partner for the expression of special foreign proteins as inclusion bodies, and the resulting, easily isolatable Polh-induced fusion inclusion bodies had almost the same characteristics as the native Polh. Here, we investigated the effects of co-expression of baculoviral FP25 protein on Polh-induced inclusion-body production in an E. coli expression system, as FP25 is known to be involved specifically in polyhedra formation. Using several analytical tools, including SDS-PAGE, pronase proteolysis, solubilization under alkaline conditions, and electron microscopy, we found that co-expressed FP25 was associated with Polh-induced inclusion bodies and that its co-expression led to formation of compact inclusion bodies as well as high production levels. We confirmed that FP-25 co-expression induced higher production levels of other heterologous protein, antimicrobial peptide Hal18, fused with aggregation-prone Polh. Therefore, co-expression of baculoviral FP25 can be promisingly used to increase the levels of baculoviral Polh-fused foreign proteins, especially harmful proteins, expressed as inclusion bodies in an E.coli expression system. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Wiley | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.subject | baculoviaral PP25 | - |
dc.subject | baculovinal polyhedrin | - |
dc.subject | inclusion body | - |
dc.subject | fusion protein | - |
dc.subject | Escherichia coli | - |
dc.subject | GREEN-FLUORESCENT PROTEIN | - |
dc.subject | AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS | - |
dc.subject | OCCLUSION-DERIVED VIRUS | - |
dc.subject | NUCLEAR-LOCALIZATION | - |
dc.subject | FUSION PARTNER | - |
dc.subject | FT-IR | - |
dc.subject | BODIES | - |
dc.subject | EXPRESSION | - |
dc.subject | GENE | - |
dc.subject | MUTATIONS | - |
dc.title | High and Compact Formation of Baculoviral. Polyhedrin-Induced Inclusion Body by Co-Expression of Baculoviral. FP25 in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.college | 해양대학원 | - |
dc.identifier.doi | 10.1002/BIT.21193 | - |
dc.author.google | Li, L | - |
dc.author.google | Kim, YS | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Seo, JH | - |
dc.author.google | Jung, HJ | - |
dc.author.google | Du, J | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 96 | - |
dc.relation.issue | 6 | - |
dc.relation.startpage | 1183 | - |
dc.relation.lastpage | 1190 | - |
dc.contributor.id | 10167197 | - |
dc.relation.journal | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.96, no.6, pp.1183 - 1190 | - |
dc.identifier.wosid | 000245459400016 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1190 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1183 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 96 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | Seo Junh hyun | - |
dc.identifier.scopusid | 2-s2.0-34247508603 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 5 | - |
dc.description.scptc | 4 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | GREEN-FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS | - |
dc.subject.keywordPlus | OCCLUSION-DERIVED VIRUS | - |
dc.subject.keywordPlus | NUCLEAR-LOCALIZATION | - |
dc.subject.keywordPlus | FUSION PARTNER | - |
dc.subject.keywordPlus | FT-IR | - |
dc.subject.keywordPlus | BODIES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordAuthor | baculoviaral PP25 | - |
dc.subject.keywordAuthor | baculovinal polyhedrin | - |
dc.subject.keywordAuthor | inclusion body | - |
dc.subject.keywordAuthor | fusion protein | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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