DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Gim young soo | - |
dc.contributor.author | Kang Dong Gyun | - |
dc.contributor.author | Kim Yoen Kyu | - |
dc.contributor.author | CHA, HYUNG JOON | - |
dc.date.accessioned | 2016-04-01T02:44:52Z | - |
dc.date.available | 2016-04-01T02:44:52Z | - |
dc.date.created | 2010-11-04 | - |
dc.date.issued | 2007-01-20 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.other | 2007-OAK-0000021802 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25694 | - |
dc.description.abstract | Mytilus galloprovincialis foot protein type-5 (Mgfp-5) is one of the mussel adhesive proteins that participate in adhesion with the substratum. We previously reported the production of recombinant Mgfp-5 in Escherichia coli and showed that the recombinant protein had superior adhesion abilities versus those of Cell-Tak, a commercially available mussel adhesive protein mixture. In the present work, we investigated the feasibility of using recombinant Mgfp-5 as a cell adhesion agent. Purified and tyrosinase-modified recombinant Mgfp-5 was used to adhere living anchorage-independent cells such as insect Drosophila S2 cells and human MOLT-4 cells onto glass slides. Our results revealed that these cell lines efficiently attached to recombinant Mgfp-5-coated glass surfaces, and that surface-immobilized S2 cells were viable and able to undergo cell division for up to 1 week. Cytochemical studies with 4',6-diamidino-2-phenylindole (DAPI) staining of nuclei and immunofluorescence for secreted foreign human erythropoietin (hEPO) from recombinant S2 cells and quantitative comparative analyses of S2 cell binding ability with Cell-Tak and poly-L-lysine, the main cell adhesion agent, were performed to demonstrate successful usage of recombinant Mgfp-5 for cell biological applications. Collectively, these results indicate that recombinant Mgfp-5 may be a useful new cell adhesion biomaterial for anchorage-independent cells. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | JOURNAL OF BIOTECHNOLOGY | - |
dc.title | Recombinant mussel adhesive protein Mgfp-5 as a cell adhesion biomaterial | - |
dc.type | Article | - |
dc.contributor.college | 해양대학원 | - |
dc.identifier.doi | 10.1016/J.JBIOTEC.2006.08.005 | - |
dc.author.google | Hwang, DS | - |
dc.author.google | Gim, Y | - |
dc.author.google | Kang, DG | - |
dc.author.google | Kim, YK | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 127 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 727 | - |
dc.relation.lastpage | 735 | - |
dc.contributor.id | 10167197 | - |
dc.relation.journal | JOURNAL OF BIOTECHNOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOTECHNOLOGY, v.127, no.4, pp.727 - 735 | - |
dc.identifier.wosid | 000243704600017 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 735 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 727 | - |
dc.citation.title | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.volume | 127 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | Kang Dong Gyun | - |
dc.contributor.affiliatedAuthor | CHA, HYUNG JOON | - |
dc.identifier.scopusid | 2-s2.0-33845668340 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 28 | - |
dc.description.scptc | 29 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TYROSINE-CONTAINING PEPTIDES | - |
dc.subject.keywordPlus | DROSOPHILA S2 CELLS | - |
dc.subject.keywordPlus | MYTILUS-EDULIS-L | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | MICROCAPSULES | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | POLYLYSINE | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | mussel adhesive protein | - |
dc.subject.keywordAuthor | recombinant adhesive | - |
dc.subject.keywordAuthor | Mgfp-5 | - |
dc.subject.keywordAuthor | cell adhesion | - |
dc.subject.keywordAuthor | cytochemistry | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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