DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, DW | - |
dc.contributor.author | Ko, HY | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Kang, H | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Song, IC | - |
dc.contributor.author | Lee, DS | - |
dc.contributor.author | Kim, S | - |
dc.date.accessioned | 2016-04-01T02:40:05Z | - |
dc.date.available | 2016-04-01T02:40:05Z | - |
dc.date.created | 2010-11-24 | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 0161-5505 | - |
dc.identifier.other | 2010-OAK-0000022036 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/25552 | - |
dc.description.abstract | The recent advances in molecular imaging techniques, using cancer-targeting nanoparticle probes, provide noninvasive tracking information on cancer cells in living subjects. Here, we report a multimodal cancer-targeted imaging system capable of concurrent fluorescence imaging, radionuclide imaging, and MRI in vivo. Methods: A cobalt-ferrite nanoparticle surrounded by fluorescent rhodamine (designated MF) within a silica shell matrix was synthesized with the AS1411 aptamer (MF-AS1411) that targets nucleolin (a cellular membrane protein highly expressed in cancer) using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC). This purified MF-AS1411 particle was bound with 2-(p-isothio-cyanatobenzyl)-1,4,7-triazacyclonane1,4,7-triacetic acid (p-SCN-bn-NOTA) chelating agent and further labeled with Ga-67-citrate (MFR-AS1411). The shape and size distribution of MFR-AS1411 were characterized by transmission electron microscope (TEM). The cellular distribution of the nucleolin protein using the MFR-AS1411 nanoparticle was detected by fluorescence confocal microscopy. Phantom MR images were obtained as the concentration of MFR-AS1411 increased, using a 1.5-T MRI scanner. In vivo Ga-67 radionuclide imaging and MRI were performed using a gamma-camera and a 1.5-T MR imager, respectively. Results: TEM imaging revealed MF and MFR-AS1411 to be spheric and well dispersed. The purified MFR-AS1411 nanoparticle showed specific fluorescence signals in nucleolin-expressing C6 cells, compared with MFR-AS1411 mutant (MFR-AS1411mt)-treated C6 cells. The rhodamine fluorescence intensity and Ga-67 activity of MFR-AS1411 were enhanced in a dose-dependent manner as the concentration of MFR-AS1411 was increased. The Ga-67 radionuclide was detected in both thighs of the mice injected with MFR-AS1411, whereas the MFR-AS1411 mutant (MFR-AS1411mt) administration revealed rapid clearance via the bloodstream, demonstrating that MFR-AS1411 specifically targeted cancer cells. Bioluminescence images in the C6 cells, stably expressing the luciferase gene, illustrated the in vivo distribution. T2-weighted MR images of the same mice injected with MFR-AS1411 showed dark T2 signals inside the tumor region, compared with the MRI signal of the tumor region injected with MFR-AS1411mt particles. Conclusion: We developed a nanoparticle-based cancer-specific imaging probe using the AS1411 aptamer in vivo and in vitro. This multimodal targeting imaging strategy, using a cancer-specific AS1411 aptamer, can be used as a versatile imaging tool for specific cancer diagnosis. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SOC NUCLEAR MEDICINE INC | - |
dc.relation.isPartOf | JOURNAL OF NUCLEAR MEDICINE | - |
dc.subject | multimodal image | - |
dc.subject | cancer targeting | - |
dc.subject | nanoparticles | - |
dc.subject | aptamer | - |
dc.subject | optical and radionuclide image | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | QUANTUM-DOT | - |
dc.subject | NANOMEDICINE | - |
dc.subject | THERAPY | - |
dc.subject | AGENT | - |
dc.subject | MICE | - |
dc.subject | PET | - |
dc.title | A Nucleolin-Targeted Multimodal Nanoparticle Imaging Probe for Tracking Cancer Cells Using an Aptamer | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.2967/JNUMED.109.069880 | - |
dc.author.google | Hwang, DW | - |
dc.author.google | Ko, HY | - |
dc.author.google | Lee, JH | - |
dc.author.google | Kang, H | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Song, IC | - |
dc.author.google | Lee, DS | - |
dc.author.google | Kim, S | - |
dc.relation.volume | 51 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 98 | - |
dc.relation.lastpage | 105 | - |
dc.contributor.id | 10069853 | - |
dc.relation.journal | JOURNAL OF NUCLEAR MEDICINE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF NUCLEAR MEDICINE, v.51, no.1, pp.98 - 105 | - |
dc.identifier.wosid | 000273263800019 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 105 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 98 | - |
dc.citation.title | JOURNAL OF NUCLEAR MEDICINE | - |
dc.citation.volume | 51 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-75149193491 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 171 | - |
dc.description.scptc | 183 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | QUANTUM-DOT | - |
dc.subject.keywordPlus | NANOMEDICINE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | AGENT | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | PET | - |
dc.subject.keywordAuthor | multimodal image | - |
dc.subject.keywordAuthor | cancer targeting | - |
dc.subject.keywordAuthor | nanoparticles | - |
dc.subject.keywordAuthor | aptamer | - |
dc.subject.keywordAuthor | optical and radionuclide image | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
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