DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, JH | - |
dc.contributor.author | Hong, WP | - |
dc.contributor.author | Yun, S | - |
dc.contributor.author | Kim, HS | - |
dc.contributor.author | Lee, JR | - |
dc.contributor.author | Park, JB | - |
dc.contributor.author | Bae, YS | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Suh, PG | - |
dc.date.accessioned | 2016-04-01T02:06:46Z | - |
dc.date.available | 2016-04-01T02:06:46Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2005-10 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.other | 2005-OAK-0000005331 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/24443 | - |
dc.description.abstract | Phospholipase C-gamma 1 (PLC-gamma 1). plays pivotal roles in cellular growth and proliferation. Upon the stimulation of growth factors and hormones, PLC-gamma 1 is rapidly phosphorylated at three known sites; Tyr(771), Tyr(783) and Tyr(1254) and its enzymatic activity is up-regulated. In this study, we demonstrate for the first time that Grb2, an adaptor protein, specifically interacts with tyrosine-phosphorylated,PLC-gamma 1 at Tyr(783). The association of Grb2 with PLC-gamma 1 was induced by the treatment with epidermal growth factor (EGF). Replacement of Tyr(783) with Phe completely blocked EGF-induced interaction of PLC-gamma 1 with Grb2, indicating that tyrosine phosphorylation of PLC-gamma 1 at Tyr783 is essential for the interaction with Grb2. Interestingly, the depletion of Grb2 from HEK-293 cells by RNA interference significantly enhanced increased EGF-induced PLC-gamma 1 enzymatic activity and mobilization of the intracellular Ca2+, while it did not affect EGF-induced tyrosine phosphorylation of PLC-gamma 1. Furthermore, overexpression of Grb2 inhibited PLC-gamma 1 enzymatic activity. Taken together, these results suggest Grb2, in addition to its key function in signaling through Ras, may have a negatively regulatory role on EGF-induced PLC-gamma 1 activation. (c) 2005 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.relation.isPartOf | CELLULAR SIGNALLING | - |
dc.subject | phospholipase c-gamma 1 | - |
dc.subject | Grb2 | - |
dc.subject | tyrosine phosphorylation | - |
dc.subject | epidermal growth factor | - |
dc.subject | interaction | - |
dc.subject | suppression | - |
dc.subject | C-GAMMA | - |
dc.subject | EGF-RECEPTOR | - |
dc.subject | NUCLEOTIDE EXCHANGE | - |
dc.subject | INSULIN-RECEPTOR | - |
dc.subject | ADAPTER PROTEIN | - |
dc.subject | RAS | - |
dc.subject | ACTIVATION | - |
dc.subject | KINASE | - |
dc.subject | ASSOCIATION | - |
dc.subject | SEQUENCE | - |
dc.title | Grb2 negatively regulates epidermal growth factor-induced phospholipase C-gamma 1 activity through the direct interaction with tyrosine-phosphorylated phospholipase C-gamma 1 | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1016/j.cellsig.2005.01.005 | - |
dc.author.google | Choi, JH | - |
dc.author.google | Hong, WP | - |
dc.author.google | Yun, S | - |
dc.author.google | Kim, HS | - |
dc.author.google | Lee, JR | - |
dc.author.google | Park, JB | - |
dc.author.google | Bae, YS | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Suh, PG | - |
dc.relation.volume | 17 | - |
dc.relation.issue | 10 | - |
dc.relation.startpage | 1289 | - |
dc.relation.lastpage | 1299 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | CELLULAR SIGNALLING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.17, no.10, pp.1289 - 1299 | - |
dc.identifier.wosid | 000231182900012 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1299 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1289 | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 17 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.identifier.scopusid | 2-s2.0-22144486622 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 17 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | C-GAMMA | - |
dc.subject.keywordPlus | EGF-RECEPTOR | - |
dc.subject.keywordPlus | NUCLEOTIDE EXCHANGE | - |
dc.subject.keywordPlus | INSULIN-RECEPTOR | - |
dc.subject.keywordPlus | ADAPTER PROTEIN | - |
dc.subject.keywordPlus | RAS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordAuthor | phospholipase c-gamma 1 | - |
dc.subject.keywordAuthor | Grb2 | - |
dc.subject.keywordAuthor | tyrosine phosphorylation | - |
dc.subject.keywordAuthor | epidermal growth factor | - |
dc.subject.keywordAuthor | interaction | - |
dc.subject.keywordAuthor | suppression | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.