DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, HS | - |
dc.contributor.author | Yumkham, S | - |
dc.contributor.author | Choi, JH | - |
dc.contributor.author | Son, GH | - |
dc.contributor.author | Kim, K | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Suh, PG | - |
dc.date.accessioned | 2016-04-01T01:49:03Z | - |
dc.date.available | 2016-04-01T01:49:03Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2006-09 | - |
dc.identifier.issn | 0022-0795 | - |
dc.identifier.other | 2006-OAK-0000006308 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/23768 | - |
dc.description.abstract | Serotonin is a neurotransmitter that alters the hypothalamic-pituitary-adrenal axis. To date, however, the molecular mechanisms underlying the role of serotonin in hormone secretion have remained largely unclear. In this study, we report that serotonin activates phospholipase C (PLC) gamma 1 in an Src-dependent manner in hypothalamic GT1-7 cells, and that pretreatment with either 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazole [3, 4-d] pyrimidine, an Src-kinase inhibitor, or U73122, a PLC inhibitor, attenuates the serotonin-induced increase in calcium levels. Also, PLC gamma 1 binds to c-Src through the Src-homology (SH) 223 domain upon serotonin treatment. Moreover, calcium increase is alleviated in the cells transiently expressing SH223 domain-deleted PLC gamma 1 or lipase inactive mutant PLC gamma 1, as compared with cells transfected with wild-type PLC gamma 1. Furthermore, the inhibition of the activities of either PLC or Src results in a significant diminution of the serotonin-induced release of gonadotropin-releasing hormone (GnRH). In addition, the results of our small-interfering RNA experiment confirm that endogenous PLC gamma 1 is a prerequisite for serotonin-mediated signaling pathways. Taken together, our findings demonstrate that serotonin stimulates the release of GnRH through the Src-PLC gamma 1 pathway, via the modulation of intracellular calcium levels. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SOC ENDOCRINOLOGY | - |
dc.relation.isPartOf | JOURNAL OF ENDOCRINOLOGY | - |
dc.subject | EPIDERMAL-GROWTH-FACTOR | - |
dc.subject | PHOSPHOLIPASE-C-GAMMA | - |
dc.subject | HORMONE NEURONS | - |
dc.subject | ANTERIOR HYPOTHALAMUS | - |
dc.subject | NEUROPEPTIDE-Y | - |
dc.subject | SRC FAMILY | - |
dc.subject | IN-VITRO | - |
dc.subject | RAT | - |
dc.subject | RECEPTOR | - |
dc.subject | PHOSPHORYLATION | - |
dc.title | Serotonin stimulates GnRH secretion through the c-Src-PLC gamma 1 pathway in GT1-7 hypothalamic cells | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1677/JOE.1.06727 | - |
dc.author.google | Kim, HS | - |
dc.author.google | Yumkham, S | - |
dc.author.google | Choi, JH | - |
dc.author.google | Son, GH | - |
dc.author.google | Kim, K | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Suh, PG | - |
dc.relation.volume | 190 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 581 | - |
dc.relation.lastpage | 591 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | JOURNAL OF ENDOCRINOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF ENDOCRINOLOGY, v.190, no.3, pp.581 - 591 | - |
dc.identifier.wosid | 000241324000003 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 591 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 581 | - |
dc.citation.title | JOURNAL OF ENDOCRINOLOGY | - |
dc.citation.volume | 190 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.identifier.scopusid | 2-s2.0-33749847235 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 15 | - |
dc.description.scptc | 16 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PHOSPHOLIPASE-C-GAMMA | - |
dc.subject.keywordPlus | ANTERIOR HYPOTHALAMUS | - |
dc.subject.keywordPlus | HORMONE-RELEASE | - |
dc.subject.keywordPlus | NEUROPEPTIDE-Y | - |
dc.subject.keywordPlus | SRC FAMILY | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
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