DC Field | Value | Language |
---|---|---|
dc.contributor.author | LEE, AH | - |
dc.contributor.author | SUH, YA | - |
dc.contributor.author | SUNG, YC | - |
dc.date.accessioned | 2016-03-31T14:27:23Z | - |
dc.date.available | 2016-03-31T14:27:23Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 1995-08-31 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 1995-OAK-0000009211 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21733 | - |
dc.description.abstract | Human immunodeficiency virus type 1 (HIV-1) contains several nonstructural genes which are required for viral replication and disease pathogenesis. Among them, rat and nef genes encode an essential transactivator of HIV-1 LTR and a pluripotent protein which seems to be essential for in vivo but not in vitro viral replication, respectively. We constructed two tat and nef defective HIV-1 and tested for their abilities Co replicate in several T cells. The defective viruses did not replicate in CD4(+) T cells, but were rescued in the recombinant Jurkat-tat cells which express the rat gene. The replication of HXB/tnC which is defective in far and nef, and contains the chloramphenicol acetyltransferase (CAT) gene was easily detected by a CAT assay. No revertant was identified during the passages of the mutant-viruses for more than two months in Jurkat-tat cells, far and nef defective HIV-1 could be used instead of wild type viruses for several purposes such as inhibitor screening and development of killed AIDS vaccine. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | TRANS-ACTIVATOR GENE | - |
dc.subject | LONG TERMINAL REPEAT | - |
dc.subject | CHLORAMPHENICOL ACETYLTRANSFERASE | - |
dc.subject | DOWN-REGULATION | - |
dc.subject | HTLV-III | - |
dc.subject | HIV-1 | - |
dc.subject | PROTEIN | - |
dc.subject | CONSTRUCTION | - |
dc.subject | ELEMENTS | - |
dc.subject | ALLELES | - |
dc.title | TAT AND NEF DEFECTIVE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REPLICATES EFFICIENTLY IN TAT-EXPRESSING T-CELLS | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.author.google | LEE, AH | - |
dc.author.google | SUH, YA | - |
dc.author.google | SUNG, YC | - |
dc.relation.volume | 5 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 306 | - |
dc.relation.lastpage | 310 | - |
dc.contributor.id | 10053752 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.5, no.4, pp.306 - 310 | - |
dc.identifier.wosid | A1995RQ85500002 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 310 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 306 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 5 | - |
dc.contributor.affiliatedAuthor | SUNG, YC | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 1 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TRANS-ACTIVATOR GENE | - |
dc.subject.keywordPlus | LONG TERMINAL REPEAT | - |
dc.subject.keywordPlus | CHLORAMPHENICOL ACETYLTRANSFERASE | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | HTLV-III | - |
dc.subject.keywordPlus | HIV-1 | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | ELEMENTS | - |
dc.subject.keywordPlus | ALLELES | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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