DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, PO | - |
dc.contributor.author | Ryu, JS | - |
dc.contributor.author | Lee, HJ | - |
dc.contributor.author | Lee, U | - |
dc.contributor.author | Park, YS | - |
dc.contributor.author | Kwak, JM | - |
dc.contributor.author | Choi, JK | - |
dc.contributor.author | Nam, HG | - |
dc.date.accessioned | 2016-03-31T14:10:28Z | - |
dc.date.available | 2016-03-31T14:10:28Z | - |
dc.date.created | 2009-03-20 | - |
dc.date.issued | 1997-06-30 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 1997-OAK-0000009814 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/21280 | - |
dc.description.abstract | Red pepper, one of the most important vegetable crops in Korea, is severely affected by viral diseases causing 20-50% reduction in product yield, A pepper strain of tobacco mosaic virus (TMV-p) is the most common virus in red pepper, To study the molecular structure of the TMV-p virus, we generated cDNA clones of the viral genome, Partial sequencing of a few cDNA clones revealed that TMV-p shares a 98% identity at the nucleotide level with the Spanish isolate of pepper mild mottle virus (PMMV-s), This suggests that TMV-p should be reclassified as the Korean isolate of PMMV (PMMV-k). The coat protein (CP) gene together with the 3' untranslated region of the PMMV-k virus was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR) using oligomers deduced from the sequence of PMMV-s, The sequence analysis of the CP gene and the 3' untranslated region further confirmed that PMMV-k is highly related to PMMV-s. The CP gene and the 3' untranslated region of PMMV-k were cloned into a plant expression vector and the construct was introduced into tobacco plants, The transgenic plants expressing the PMMV-k CP gene were delayed in developing systemic disease or failed to develop symptoms at all after inoculation with PMMV-k. Delay of symptoms was also observed when the plants were inoculated with TMV-OM which shares a 74% homology with PMMV-k in the amino acid sequence of the CP region, In a local lesion host, the CP expressing plants exhibited a greatly reduced number of necrotic lesions as compared to control plants after inoculation with TMV-OM, Our results show that CP-mediated viral resistance is readily applicable in the case of PMMV-k and can provide resistance to other viruses in the tobamovirus group. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MOLECULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | BANDING MOSAIC-VIRUS | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | ANTISENSE RNA | - |
dc.subject | POTYVIRUS | - |
dc.subject | PROTECTION | - |
dc.subject | INFECTION | - |
dc.title | Resistance to tobamoviruses in transgenic tobacco plants expressing the coat protein gene of pepper mild mottle virus (Korean isolate) | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.author.google | Lim, PO | - |
dc.author.google | Ryu, JS | - |
dc.author.google | Lee, HJ | - |
dc.author.google | Lee, U | - |
dc.author.google | Park, YS | - |
dc.author.google | Kwak, JM | - |
dc.author.google | Choi, JK | - |
dc.author.google | Nam, HG | - |
dc.relation.volume | 7 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 313 | - |
dc.relation.lastpage | 319 | - |
dc.contributor.id | 10087591 | - |
dc.relation.journal | MOLECULES AND CELLS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.7, no.3, pp.313 - 319 | - |
dc.identifier.wosid | A1997XG80200002 | - |
dc.date.tcdate | 2018-12-01 | - |
dc.citation.endPage | 319 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 313 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 7 | - |
dc.contributor.affiliatedAuthor | Nam, HG | - |
dc.identifier.scopusid | 2-s2.0-0031591543 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 6 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BANDING MOSAIC-VIRUS | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | ANTISENSE RNA | - |
dc.subject.keywordPlus | POTYVIRUS | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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