DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, JS | - |
dc.contributor.author | Lee, S | - |
dc.contributor.author | Jung, KH | - |
dc.contributor.author | Jun, SH | - |
dc.contributor.author | Kim, C | - |
dc.contributor.author | An, G | - |
dc.date.accessioned | 2016-03-31T13:29:02Z | - |
dc.date.available | 2016-03-31T13:29:02Z | - |
dc.date.created | 2009-08-06 | - |
dc.date.issued | 2000-07 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.other | 2000-OAK-0000001438 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19927 | - |
dc.description.abstract | The genomic clone encoding an ol-tubulin, OsTubA1, has been isolated from rice (Oryza sativa L.). The gene consists of four exons and three introns. RNA-blot analysis showed that the gene is strongly expressed in actively dividing tissues, including root tips, young leaves, and young flowers. Analysis of chimeric fusions between OsTubA1 and beta-glucuronidase (GLIS) revealed that the intron 1 was required for high-level GUS expression in actively dividing tissues, corresponding with normal expression pattern of OsTubA1. Fusion constructs lacking the intron 1 showed more GUS staining in mature tissues rather than young tissues. When the intron 1 was placed at the distal region from S'-upstream region or at the 3'-untranslated region, no enhancement of GUS expression was observed. Sequential deletions of the OsTubA1 intron 1 brought about a gradual reduction of GUS activity in calli. These results suggest that tissue-preferential expression of the OsTubA1 gene is mediated by the intron 1 and that it may be involved in a mechanism for an efficient RNA splicing that is position dependent. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC PLANT PHYSIOLOGISTS | - |
dc.relation.isPartOf | PLANT PHYSIOLOGY | - |
dc.subject | ZEA-MAYS L | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | SPLICING EFFICIENCY | - |
dc.subject | TRANSCRIBED REGION | - |
dc.subject | BETA-GLUCURONIDASE | - |
dc.subject | TRANSGENIC TOBACCO | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | MAIZE INTRON | - |
dc.subject | PROMOTER | - |
dc.subject | SEQUENCES | - |
dc.title | Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1104/pp.123.3.1005 | - |
dc.author.google | Jeon, JS | - |
dc.author.google | Lee, S | - |
dc.author.google | Jung, KH | - |
dc.author.google | Jun, SH | - |
dc.author.google | Kim, C | - |
dc.author.google | An, G | - |
dc.relation.volume | 123 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 1005 | - |
dc.relation.lastpage | 1014 | - |
dc.contributor.id | 10184525 | - |
dc.relation.journal | PLANT PHYSIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLANT PHYSIOLOGY, v.123, no.3, pp.1005 - 1014 | - |
dc.identifier.wosid | 000088213300023 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1014 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1005 | - |
dc.citation.title | PLANT PHYSIOLOGY | - |
dc.citation.volume | 123 | - |
dc.contributor.affiliatedAuthor | An, G | - |
dc.identifier.scopusid | 2-s2.0-0033928910 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 92 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ZEA-MAYS L | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | SPLICING EFFICIENCY | - |
dc.subject.keywordPlus | TRANSCRIBED REGION | - |
dc.subject.keywordPlus | BETA-GLUCURONIDASE | - |
dc.subject.keywordPlus | TRANSGENIC TOBACCO | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | MAIZE INTRON | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.