DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, MJ | - |
dc.contributor.author | Chang, JS | - |
dc.contributor.author | Park, SK | - |
dc.contributor.author | Hwang, JI | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Suh, PG | - |
dc.date.accessioned | 2016-03-31T13:28:43Z | - |
dc.date.available | 2016-03-31T13:28:43Z | - |
dc.date.created | 2009-08-12 | - |
dc.date.issued | 2000-07-25 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.other | 2000-OAK-0000001457 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/19915 | - |
dc.description.abstract | A recent report that microinjection of the SH3 domain of PLC-gamma 1 could induce DNA synthesis raised the functional importance of the SH3 domain of PLC-gamma 1 in mitogenic signaling. In this report, we provide evidence that SOS1, a p2Ras-specific guanine nucleotide exchange factor, directly binds to the SH3 domain of PLC-gamma 1, and that the SH3 domain of 1 is involved in SOS1-mediated p21Ras activation. SOS1 was coprecipitated with the GST-fused SH3 domain of PLC-gamma 1 in vitro. The interaction between SOS1 and the PLC-gamma 1 SH3 domain is mediated by direct physical interaction. The carboxyl-terminal proline-rich domain of SOS1 is involved in the interaction with the PLC-gamma 1 SH3 domain. Moreover, PLC-gamma 1 could be co-immunoprecipitated with SOS1 antibody in cell lysates. From transient expression studies, we could demonstrate that the SH3 domain of PLC-gamma 1 is necessary for the association with SOS1 in vivo. Intriguingly, overexpression of the SH3 domain of PLC-gamma 1, lipase-inactive PLC-gamma 1, or wild-type PLC-gamma 1 elevated p21Ras activity and ERK activity when compared with vector transfected cells. The PLC-gamma 1 mutant lacking the SH3 domain could not activate p21Ras. p21Ras activities in cell lines overexpressing either PLC-gamma 1 or the SH2-SH2-SH3 domain of PLC-gamma 1 were elevated about 2-fold compared to vector transfected cells. This study is the first to demonstrate that the PLC-gamma 1 SH3 domain enhances p21Ras activity, and that the SH3 domain of PLC-gamma 1 may be involved in the SOS1-mediated signaling pathway. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | BIOCHEMISTRY | - |
dc.subject | TYROSINE KINASE | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | INOSITOL TRISPHOSPHATE | - |
dc.subject | SIGNAL TRANSDUCTION | - |
dc.subject | ELEVATED CONTENT | - |
dc.subject | DNA-SYNTHESIS | - |
dc.subject | FACTOR HSOS1 | - |
dc.subject | C ISOZYMES | - |
dc.subject | GRB2 | - |
dc.subject | RECEPTOR | - |
dc.title | Direct interaction of SOS1 ras exchange protein with the SH3 domain of phospholipase C-gamma 1 | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1021/bi992558t | - |
dc.author.google | Kim, MJ | - |
dc.author.google | Chang, JS | - |
dc.author.google | Park, SK | - |
dc.author.google | Hwang, JI | - |
dc.author.google | Ryu, SH | - |
dc.author.google | Suh, PG | - |
dc.relation.volume | 39 | - |
dc.relation.issue | 29 | - |
dc.relation.startpage | 8674 | - |
dc.relation.lastpage | 8682 | - |
dc.contributor.id | 10052640 | - |
dc.relation.journal | BIOCHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCHEMISTRY, v.39, no.29, pp.8674 - 8682 | - |
dc.identifier.wosid | 000088376900037 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 8682 | - |
dc.citation.number | 29 | - |
dc.citation.startPage | 8674 | - |
dc.citation.title | BIOCHEMISTRY | - |
dc.citation.volume | 39 | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.identifier.scopusid | 2-s2.0-0001550680 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 41 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TYROSINE KINASE | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | INOSITOL TRISPHOSPHATE | - |
dc.subject.keywordPlus | SIGNAL TRANSDUCTION | - |
dc.subject.keywordPlus | ELEVATED CONTENT | - |
dc.subject.keywordPlus | DNA-SYNTHESIS | - |
dc.subject.keywordPlus | FACTOR HSOS1 | - |
dc.subject.keywordPlus | C ISOZYMES | - |
dc.subject.keywordPlus | GRB2 | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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