DC Field | Value | Language |
---|---|---|
dc.contributor.author | Back, SH | - |
dc.contributor.author | Shin, SJ | - |
dc.contributor.author | Jang, SK | - |
dc.date.accessioned | 2016-03-31T13:03:24Z | - |
dc.date.available | 2016-03-31T13:03:24Z | - |
dc.date.created | 2009-08-19 | - |
dc.date.issued | 2002-07-26 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2002-OAK-0000002800 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/18970 | - |
dc.description.abstract | The polypyrimidine tract-binding protein (PTB), an RNA-binding protein, is required for efficient translation of some mRNAs containing internal ribosomal entry sites (IRESs). Here we provide evidence that the addition of apoptosis-inducing agents to cells results in the cleavage of PTB isoforms 1, 2, and 4 by caspase-3. This cleavage of PTB separated the N-terminal region, containing NLS-RRM1, from the C-terminal region, containing RRM2-3-4. Our data indicate that there are three noncanonical caspase-3 target sites in PTBs, namely Ile-Val-Pro-Asp(7) down arrow Ile, Leu-Tyr-Thr-Asp(139) down arrow Ser, and Ala-Ala-Val-Asp(172) down arrow Ala. The C-terminal PTB fragments localized to the cytoplasm, as opposed to the nucleus where most intact PTBs are found. Moreover, these G terminal PTB fragments inhibited translation of polio-viral mRNA, which contains an IRES element requiring PTB for its activation. This suggests that translation of some IRES-containing mRNAs is regulated by proteolytic cleavage of PTB during apoptosis. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | RIBOSOME-ENTRY-SITE | - |
dc.subject | INITIATION-FACTOR 4G | - |
dc.subject | MOUTH-DISEASE VIRUS | - |
dc.subject | CAP-INDEPENDENT TRANSLATION | - |
dc.subject | 5&apos | - |
dc.subject | NONTRANSLATED REGION | - |
dc.subject | PROGRAMMED CELL-DEATH | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | INTERNAL INITIATION | - |
dc.subject | FUNCTIONAL REQUIREMENT | - |
dc.subject | MEDIATED TRANSLATION | - |
dc.title | Polypyrimidine tract-binding proteins are cleaved by caspase-3 during apoptosis | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/jbc.M203887200 | - |
dc.author.google | Back, SH | - |
dc.author.google | Shin, SJ | - |
dc.author.google | Jang, SK | - |
dc.relation.volume | 277 | - |
dc.relation.issue | 30 | - |
dc.relation.startpage | 27200 | - |
dc.relation.lastpage | 27209 | - |
dc.contributor.id | 10088382 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.277, no.30, pp.27200 - 27209 | - |
dc.identifier.wosid | 000177055900065 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 27209 | - |
dc.citation.number | 30 | - |
dc.citation.startPage | 27200 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 277 | - |
dc.contributor.affiliatedAuthor | Jang, SK | - |
dc.identifier.scopusid | 2-s2.0-0037178840 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 33 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RIBOSOME-ENTRY-SITE | - |
dc.subject.keywordPlus | INITIATION-FACTOR 4G | - |
dc.subject.keywordPlus | MOUTH-DISEASE VIRUS | - |
dc.subject.keywordPlus | CAP-INDEPENDENT TRANSLATION | - |
dc.subject.keywordPlus | 5&apos | - |
dc.subject.keywordPlus | NONTRANSLATED REGION | - |
dc.subject.keywordPlus | PROGRAMMED CELL-DEATH | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | INTERNAL INITIATION | - |
dc.subject.keywordPlus | FUNCTIONAL REQUIREMENT | - |
dc.subject.keywordPlus | MEDIATED TRANSLATION | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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