Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case
SCIE
SCOPUS
- Title
- Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case
- Authors
- Lee, S; Kim, J; Son, JS; Nam, J; Jeong, DH; Lee, K; Jang, S; Yoo, J; Lee, J; Lee, DY; Kang, HG; An, GH
- Date Issued
- 2003-12
- Publisher
- OXFORD UNIV PRESS
- Abstract
- We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been identified from rice sequence databases. Our PCR screening for T-DNA insertions within 12 MADS-box genes resulted in the identification of five insertions in four different genes. These DNA pools will be valuable when isolating T-DNA insertional mutants in various rice genes. The DNA pool screening service and the mutant seeds are available upon request to genean@postecb.ac.kr.
- Keywords
- insertional mutagenesis; MADS-box genes; pool screening; rice; T-DNA; FLORAL ORGAN IDENTITY; INSERTIONAL MUTAGENESIS; ECTOPIC EXPRESSION; FLOWER DEVELOPMENT; ARABIDOPSIS-THALIANA; OVULE DEVELOPMENT; DRAFT SEQUENCE; TRANSCRIPTION; FAMILY; PLANTS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18177
- DOI
- 10.1093/pcp/pcg156
- ISSN
- 0032-0781
- Article Type
- Article
- Citation
- PLANT AND CELL PHYSIOLOGY, vol. 44, no. 12, page. 1403 - 1411, 2003-12
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