DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, CH | - |
dc.contributor.author | You, JH | - |
dc.contributor.author | Kang, HG | - |
dc.contributor.author | Hur, JH | - |
dc.contributor.author | Kim, YH | - |
dc.contributor.author | Han, MJ | - |
dc.contributor.author | An, KS | - |
dc.contributor.author | Chung, BC | - |
dc.contributor.author | Lee, CH | - |
dc.contributor.author | An, GH | - |
dc.date.accessioned | 2016-03-31T12:17:23Z | - |
dc.date.available | 2016-03-31T12:17:23Z | - |
dc.date.created | 2009-08-06 | - |
dc.date.issued | 2004-03 | - |
dc.identifier.issn | 0167-4412 | - |
dc.identifier.other | 2004-OAK-0000004484 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/17753 | - |
dc.description.abstract | We have developed a binary T-DNA vector, pGA2717, that contains the promoter-less beta-glucuronidase (gus) gene adjacent to the right border and the promoter-less green fluorescence protein (gfp) gene next to the left border of the T-DNA. Therefore, inserting T-DNA into a gene can result in the activation of either gus or gfp. A total of 12 169 T-DNA insertional lines of japonica rice were generated using this binary vector. Out of 3140 lines examined, 0.5% of their mature seeds and 2.0% of the 3-day-old etiolated seedlings were GFP-positive. However, GUS assays of the same materials resulted in the identification of 151 (4.8%) GUS-positive lines. Using DNA gel blot and reverse transcription (RT)-PCR analyses, we confirmed that the GFP-positive lines were a true indication of gene trapping. A fusion transcript was also obtained between gfp and the trapped gene. We isolated 990 genomic sequences flanking T-DNA from our analysis of 2099 transgenic plants. Among the insertions, 625 T-DNAs were integrated into genic regions; 361 were located in intergenic regions. These tagging lines will be valuable in trapping and studying various genes for their expression patterns, as well as providing a useful tool for genetic approaches. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.relation.isPartOf | PLANT MOLECULAR BIOLOGY | - |
dc.subject | flanking sequence database | - |
dc.subject | gene trap | - |
dc.subject | GFP | - |
dc.subject | GUS | - |
dc.subject | insertional mutagenesis | - |
dc.subject | T-DNA | - |
dc.subject | GREEN-FLUORESCENT PROTEIN | - |
dc.subject | AGROBACTERIUM-MEDIATED TRANSFORMATION | - |
dc.subject | MULTIPLE COMPLEX PATTERNS | - |
dc.subject | TRANSGENIC RICE PLANTS | - |
dc.subject | BINARY VECTOR | - |
dc.subject | VITAL MARKER | - |
dc.subject | ILLEGITIMATE RECOMBINATION | - |
dc.subject | ARABIDOPSIS GENOME | - |
dc.subject | FIREFLY LUCIFERASE | - |
dc.subject | BACKBONE SEQUENCES | - |
dc.title | Generation of T-DNA tagging lines with a bidirectional gene trap vector and the establishment of an insertion-site database | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1023/B:PLAN.0000038257.93381.05 | - |
dc.author.google | Ryu, CH | - |
dc.author.google | You, JH | - |
dc.author.google | Kang, HG | - |
dc.author.google | Hur, JH | - |
dc.author.google | Kim, YH | - |
dc.author.google | Han, MJ | - |
dc.author.google | An, KS | - |
dc.author.google | Chung, BC | - |
dc.author.google | Lee, CH | - |
dc.author.google | An, GH | - |
dc.relation.volume | 54 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 489 | - |
dc.relation.lastpage | 502 | - |
dc.contributor.id | 10184525 | - |
dc.relation.journal | PLANT MOLECULAR BIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLANT MOLECULAR BIOLOGY, v.54, no.4, pp.489 - 502 | - |
dc.identifier.wosid | 000223358600002 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 502 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 489 | - |
dc.citation.title | PLANT MOLECULAR BIOLOGY | - |
dc.citation.volume | 54 | - |
dc.contributor.affiliatedAuthor | An, GH | - |
dc.identifier.scopusid | 2-s2.0-4444297609 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 63 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | GREEN-FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | AGROBACTERIUM-MEDIATED TRANSFORMATION | - |
dc.subject.keywordPlus | MULTIPLE COMPLEX PATTERNS | - |
dc.subject.keywordPlus | TRANSGENIC RICE PLANTS | - |
dc.subject.keywordPlus | BINARY VECTOR | - |
dc.subject.keywordPlus | VITAL MARKER | - |
dc.subject.keywordPlus | ILLEGITIMATE RECOMBINATION | - |
dc.subject.keywordPlus | ARABIDOPSIS GENOME | - |
dc.subject.keywordPlus | FIREFLY LUCIFERASE | - |
dc.subject.keywordPlus | BACKBONE SEQUENCES | - |
dc.subject.keywordAuthor | flanking sequence database | - |
dc.subject.keywordAuthor | gene trap | - |
dc.subject.keywordAuthor | GFP | - |
dc.subject.keywordAuthor | GUS | - |
dc.subject.keywordAuthor | insertional mutagenesis | - |
dc.subject.keywordAuthor | T-DNA | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
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