DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, ES | - |
dc.contributor.author | Namgyu Lee | - |
dc.contributor.author | Park, JW | - |
dc.contributor.author | Choi, KY | - |
dc.date.accessioned | 2016-03-31T08:22:20Z | - |
dc.date.available | 2016-03-31T08:22:20Z | - |
dc.date.created | 2013-11-12 | - |
dc.date.issued | 2013-10-11 | - |
dc.identifier.issn | 0957-4484 | - |
dc.identifier.other | 2013-OAK-0000028531 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15156 | - |
dc.description.abstract | We analyzed the enzymatic profiles of on-chip DNA ligation as we controlled the lateral spacing of surface-immobilized DNA substrates using dendron molecules with different sizes at the nanoscale. Enzymatic on-chip DNA ligation was performed on the dendron-coated surface within 20 min with no need for post-ligation gel electrophoresis. The enzymatic DNA repair was assessed by the fluorescence intensity at the repaired DNA duplex after thermally dissociating the unligated Cy3-labeled DNA from the DNA duplex, in which the Cy3-labeled DNA was hybridized prior to the on-chip DNA ligation. The rate of the nick-sealing reaction on the 27-acid dendron surface was 3-fold higher than that on the 9-acid dendron surface, suggesting that the wider lateral spacing determined by the larger dendron molecule could facilitate the access of DNA ligase to the nick site. The performance of on-chip DNA ligation was dropped to 10% and 3% when the nick was replaced by one-and two-nucleotide-long gaps, respectively. The 5 0 terminal phosphorylation of DNA strands by polynucleotide kinase and the on-chip DNA cleavage by endonucleases were also quantitatively monitored throughout the on-chip DNA ligation on the dendron-coated surface. A better understanding of the enzymatic kinetics of on-chip DNA ligation will contribute to a more reliable performance of various on-chip DNA ligation-based assays. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | IOP science | - |
dc.relation.isPartOf | Nanotechnology | - |
dc.title | Kinetic Characterization of On-Chip DNA ligation on Dendron-coated surfaces with Nanoscaled lateral spacing | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1088/0957-4484/24/40/405703 | - |
dc.author.google | Kim, ES | - |
dc.author.google | Lee, N | - |
dc.author.google | Park, JW | - |
dc.author.google | Choi, KY | - |
dc.relation.volume | 24 | - |
dc.relation.issue | 40 | - |
dc.contributor.id | 10053210 | - |
dc.relation.journal | Nanotechnology | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Nanotechnology, v.24, no.40, pp.1 - 10 | - |
dc.identifier.wosid | 000324516300016 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 10 | - |
dc.citation.number | 40 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Nanotechnology | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Park, JW | - |
dc.contributor.affiliatedAuthor | Choi, KY | - |
dc.identifier.scopusid | 2-s2.0-84884245410 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 1 | - |
dc.description.scptc | 1 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | POINT MUTATIONS | - |
dc.subject.keywordPlus | POLYNUCLEOTIDE KINASE | - |
dc.subject.keywordPlus | RNA LIGASES | - |
dc.subject.keywordPlus | T4 DNA | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
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