DC Field | Value | Language |
---|---|---|
dc.contributor.author | Akter, Shammi | - |
dc.contributor.author | Afrin, Shajia | - |
dc.contributor.author | Kim, Jaeyoon | - |
dc.contributor.author | Kang, Joohyun | - |
dc.contributor.author | Razzak, Md Abdur | - |
dc.contributor.author | Berggren, Per-Olof | - |
dc.contributor.author | Hwang, Inhwan | - |
dc.date.accessioned | 2023-03-02T09:20:31Z | - |
dc.date.available | 2023-03-02T09:20:31Z | - |
dc.date.created | 2023-03-02 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/116327 | - |
dc.description.abstract | <jats:p>GLP-1 (Glucagon-like peptide-1) is a peptide that stimulates insulin secretion from the β-cell for glycemic control of the plasma blood glucose level. Its mimetic exenatide (synthetic Exendin-4) with a longer half-life of approximately 3.3–4 h is widely used in clinical application to treat diabetes. Currently, exenatide is chemically synthesized. In this study, we report that the GLP-1 analogue recombinant Exendin-4 (Exdn-4) can be produced at a high level in <jats:italic>Nicotiana benthamiana</jats:italic>, with an estimated yield of 50.0 µg/g fresh biomass. For high-level expression, we generated a recombinant gene, <jats:italic>B:GB1:ddCBD1m:8xHis : Exendin-4</jats:italic> (<jats:italic>BGC : Exdn-4</jats:italic>), for the production of Exendin-4 using various domains such as the BiP signal peptide, the GB1 domain (B1 domain of streptococcal G protein), a double cellulose binding domain 1 (CBD1), and 8 His residues (8xHis) to the N-terminus of Exendin-4. GB1 was used to increase the expression, whereas double CBD1 and 8xHis were included as affinity tags for easy purification using MCC beads and Ni<jats:sup>2+</jats:sup>-NTA resin, respectively. BGC : Exdn-4 was purified by single-step purification to near homogeneity using both Ni<jats:sup>2+</jats:sup>-NTA resin and microcrystalline cellulose (MCC) beads. Moreover, Exdn-4 without any extra residues was produced from BGC : Exdn-4 bound onto MCC beads by treating with enterokinase. Plant-produced Exdn-4 (Exendin-4) was as effective as chemically synthesized Exendin-4 in glucose-induced insulin secretion (GIIS) from mouse MIN6m9 cells a pancreatic beta cell line.</jats:p> | - |
dc.language | English | - |
dc.publisher | Frontiers Media S.A. | - |
dc.relation.isPartOf | Frontiers in Plant Science | - |
dc.title | Production of active Exendin-4 in Nicotiana benthamiana and its application in treatment of type-2 diabetics | - |
dc.type | Article | - |
dc.identifier.doi | 10.3389/fpls.2022.1062658 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Frontiers in Plant Science, v.13 | - |
dc.identifier.wosid | 000907484200001 | - |
dc.citation.title | Frontiers in Plant Science | - |
dc.citation.volume | 13 | - |
dc.contributor.affiliatedAuthor | Hwang, Inhwan | - |
dc.identifier.scopusid | 2-s2.0-85145508710 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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