DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, SY | - |
dc.contributor.author | Park, JH | - |
dc.contributor.author | Kim, JS | - |
dc.date.accessioned | 2022-01-10T07:00:13Z | - |
dc.date.available | 2022-01-10T07:00:13Z | - |
dc.date.created | 2021-07-05 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/109136 | - |
dc.description.abstract | The human activating signal cointegrator 1 (ASC-1) homology (ASCH) domain is frequently observed in many organisms, although its function has not yet been clearly defined. In Zymomonas mobilis ZM4, the ZMO0922 gene encodes a polypeptide that includes an ASCH domain (zmASCH). To provide a better structural background for the probable role of ASCH domain-containing proteins, the ZMO0922 gene was cloned and expressed. The purified protein was crystallized from 30%(w/v) polyethylene glycol 400, 0.1 M cacodylic acid pH 6.5 and 0.2 M lithium sulfate. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. The crystal belonged to the primitive trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 51.67, c = 207.30 A, alpha = beta = 90, gamma = 120 degrees. Assuming the presence of one molecule in the asymmetric unit gave a Matthews coefficient of 4.69 A3 Da-1, corresponding to a solvent content of 73.7%. | - |
dc.language | English | - |
dc.publisher | International Union of Crystallography | - |
dc.relation.isPartOf | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | - |
dc.title | Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of an ASCH domain-containing protein from Zymomonas mobilis ZM4 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1107/S1744309110053467 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v.67, no.3, pp.310 - 312 | - |
dc.identifier.wosid | 000288225100004 | - |
dc.citation.endPage | 312 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 310 | - |
dc.citation.title | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | - |
dc.citation.volume | 67 | - |
dc.contributor.affiliatedAuthor | Park, SY | - |
dc.identifier.scopusid | 2-s2.0-79952649252 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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