Bacterial and methanogenic archaeal communities during the single-stage anaerobic digestion of high-strength food wastewater

Abstract

Single-stage anaerobic digestion (AD) was operated to treat high-strength food wastewater (FWW) derived from food waste recycling facilities at two different organic loading rates (OLRs) of 3.5 (Phase I) and 7 (Phase II) kg COD/m(3) d. Changes in composition of microbial communities were investigated using quantitative real-time PCR (qPCR) and barcoded-pyrosequencing. At the high FWW loading rate, AD showed efficient performance (i. e., organic matter removal and methane production). Bacterial communities were represented by the phyla Bacteroidetes, Firmicutes, Synergistetes and Actinobacteria. During the entire digestion process, the relative abundance phylum Chloroflexi decreased significantly. The qPCR analysis demonstrated that the methanogenic communities shifted from aceticlastic (Methanosarcinales) to hydrogenotrophic methanogens (Methanobacteriales and Methanomicrobiales) with high increase in the proportion of syntrophic bacterial communities. Canonical correspondence analysis revealed a strong relationship between reactor performance and microbial community shifts. (C) 2014 Elsevier Ltd. All rights reserved.