DC Field | Value | Language |
---|---|---|
dc.contributor.author | JEON, SANGMIN | - |
dc.contributor.author | LEE, HYEONJEONG | - |
dc.contributor.author | KIM, GWANHO | - |
dc.contributor.author | PARK, EUNJIN | - |
dc.date.accessioned | 2020-03-12T12:50:04Z | - |
dc.date.available | 2020-03-12T12:50:04Z | - |
dc.date.created | 2020-03-12 | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/101378 | - |
dc.description.abstract | We have developed a method for rapid detection of pathogenic bacteria from water using a virtual net comprising magnetic nanoparticle clusters (MNC). When an external magnetic field was applied to the antibody-functionalized MNC (Ab-MNC) solution in a glass tube (GT), the Ab-MNCs were aligned along the direction of the applied magnetic field to form a wall of MNCs. The injection of a liquid into the GT pushed the MNCs to flow when the drag force exceeded the magnetic force that held the MNCs. In contrast, injection of a liquid into the GT wrapped with a copper tape (Cu-GT) created a magnetic field in the opposite direction of the liquid flow according to Lenz's law, which retained the MNCs inside Cu-GT even at a flow rate 2.5 times higher than the maximum flow rate at which the MNCs were retained inside the GT. As proof of concept, E. coli O157:H7-spiked aqueous solutions were injected into Cu-GT containing Ab-MNCs. The structural flexibility of the Ab-MNC wall allowed the liquid to pass through but induced binding of the bacteria to the Ab-MNC wall, just as the wall acted like a virtual net. The detection limit was 10(2) CFU/mL of E. coli as measured by an ATP luminometer, and the total assay time was 15 min including 10 min for the isolation and separation steps. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.title | Lenz’s Law-Based Virtual Net for Detection of Pathogenic Bacteria from Water | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.9b03636 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.91, no.24, pp.15585 - 15590 | - |
dc.identifier.wosid | 000503910600035 | - |
dc.citation.endPage | 15590 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 15585 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 91 | - |
dc.contributor.affiliatedAuthor | JEON, SANGMIN | - |
dc.contributor.affiliatedAuthor | LEE, HYEONJEONG | - |
dc.contributor.affiliatedAuthor | KIM, GWANHO | - |
dc.contributor.affiliatedAuthor | PARK, EUNJIN | - |
dc.identifier.scopusid | 2-s2.0-85075649488 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI O157 | - |
dc.subject.keywordPlus | SALMONELLA-TYPHIMURIUM | - |
dc.subject.keywordPlus | LISTERIA-MONOCYTOGENES | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CAPTURE | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | BEAD | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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