Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
SCIE
SCOPUS
- Title
- Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
- Authors
- Reddy, H.K.N; SONG, CHANGYONG; Yoon, C.H.; Aquila, A; Ayver, K.; Awel, S.; Bernstern, P.; Bieleki, J.; Bobkov, S.; Bucher, M.; Carini, G.A.; Caroon. S.; Chapman, H; Daurer, B.; DeMirci, H.; Ekeberg, T; Fromme, P.; Hadju, J.; Hanke, M. F.; Hart, P; Hogue, B.G; Hosseinizadeh, A.; Kim, Yoonhee; Kirian, R; Larson, D.; Nettelblad, D.; Ourmazd, A; Rose, M.; Schwander, P; Seibert, M.; Selberg, J; Spence, J; Loh, D; Svender, M; Gijs Van der Schot; Vartanyants, I; Williams, G; Maia, F; Xavier, L; Mancuso, A; Mühlig, K; Nam, D; Munke, A
- Date Issued
- 2017-07
- Publisher
- Nature Publishing Company
- Abstract
- Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported similar to 600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/98616
- DOI
- 10.1038/sdata.2017.79
- ISSN
- 2052-4463
- Article Type
- Article
- Citation
- Scientific Data, vol. 4, page. 170079, 2017-07
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.