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Bioinspired Load-Bearing Hydrogel Based on Engineered Sea Anemone Skin-Derived Collagen-Like Protein

Title
Bioinspired Load-Bearing Hydrogel Based on Engineered Sea Anemone Skin-Derived Collagen-Like Protein
Authors
Yang, Yun JungChoi, Yoo SeongCha, Hyung Joon
Date Issued
2018-12
Publisher
WILEY-V C H VERLAG GMBH
Abstract
With the help of recombinant DNA technology, many protein candidates have been investigated and engineered for biomaterial applications. Particularly, several repeat sequences with unique secondary structures have been selected as minimal building blocks for biosynthesis to improve the mechanical properties of biomaterials. However, most of these structural proteins have been limited to silk, elastin, collagen, and resilin for decades. In the present work, new repeat sequence found in sea anemone are characterized and biosynthesized into a recombinant protein (named anegen) for potential use as a load-bearing biomaterial. Because its repeat sequence unit has a unique polyproline II structure, which is prevalently found in the triple-helix of collagen, it is assumed to be a promising structural protein candidate that can provide conformational flexibility and elasticity. Because anegen has approximate to 10% tyrosine residues, inspiration is taken from di-tyrosine crosslinking in the hinge structures of insects, which can be initiated by light activation. It is found that the anegen hydrogel shows higher mechanical properties than a gelatin hydrogel and endures a compression series without deformation. Moreover, the mechanical properties of the anegen hydrogel are controllable through different crosslinking conditions in a wide range of material applications. Importantly, the anegen hydrogel exhibited suitable cell retainability and cell morphology as an implantable biomaterial. Thus, based on its mechanical properties and biocompatibility, the anegen hydrogel can be used as a potential load-bearing and cell-loading scaffolding biomaterial in the tissue and biomedical engineering fields.
URI
https://oasis.postech.ac.kr/handle/2014.oak/95458
ISSN
1860-6768
Article Type
Article
Citation
BIOTECHNOLOGY JOURNAL, vol. 13, no. 12, page. 1800086, 2018-12
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차형준CHA, HYUNG JOON
Dept. of Chemical Enginrg
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