Controls of Methylmercury Bioaccumulation in Forest Floor Food Webs

Abstract

Compared to the extensive research on aquatic ecosystems, very little is known about the sources and trophic transfer of methylmercury (MeHg) in terrestrial ecosystems. In this study, we examine energy flow and trophic structure using stable carbon (delta C-13) and nitrogen (delta N-15) isotope ratios, respectively, and MeHg levels in basal resources and terrestrial invertebrates from four temperate forest ecosystems. We show that MeHg levels in biota increased significantly (p < 0.01) with delta C-13 and delta N-15 at all sites, implying the importance of both microbially processed diets (with increased delta C-13) and trophic level (with increased delta N-15) at which organisms feed, on MeHg levels in forest floor biota. The trophic magnification slopes of MeHg (defined as the slope of log(10)MeHg vs delta N-15) for these forest floor food webs (0.200.28) were not significantly different (p > 0.05) from those observed for diverse temperate freshwater systems (0.24 +/- 0.07; n = 78), demonstrating for the first time the nearly equivalent efficiencies with which MeHg moves up the food chain in these contrasting ecosystem types. Our results suggest that in situ production of MeHg within the forest floor and efficient biomagnification both elevate MeHg levels in carnivorous invertebrates in temperate forests, which can contribute to significant bioaccumulation of this neurotoxin in terrestrial apex predators.