HCRs, anti-crossover proteins restrict crossover number in Arabidopsis
- Title
- HCRs, anti-crossover proteins restrict crossover number in Arabidopsis
- Authors
- 김주현; 김한울; 김재일; 김언정; 박지혜; 임안희; CHOI, KYUHA
- Date Issued
- 2018-07-11
- Publisher
- 한국육종학회
- Abstract
- Meiotic crossover recombination affects genetic diversity in population and is a critical tool for breeding. During meiosis, meiotic DSBs are repaired to reciprocal crossovers through class I and class II pathways. Anti-crossover factors such as FANCM, RECQ4 and FIGL1 were known to restrict crossovers in class II pathway dependent manner. However, the mechanism underlining how crossover number is limited to one to three along chromosome remains unexplored. We performed a high throughput genetic screening of higher crossover rate (hcr) mutants by using fluorescent seed-based system, enabling the measurement of crossover frequency in individual plants. We found that hcr mutants (hcr1, hcr2, hcr3) were new anti-crossover mutants by genetic analysis and deep-sequencing. We present characterization of hcr2 and hcr3 mutants, including mapping of them and their effects on crossover frequency in defined intervals along chromosomes.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94821
- Article Type
- Conference
- Citation
- 2018 한국육종학회-차세대BG21사업-GSP사업 공동심포지엄, 2018-07-11
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