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Cited 8 time in webofscience Cited 19 time in scopus
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Specific discrimination of three pathogenic salmonella enterica subsp enterica serotypes using CarB-based oligonuceotide microarray

Title
Specific discrimination of three pathogenic salmonella enterica subsp enterica serotypes using CarB-based oligonuceotide microarray
Authors
Shin, HHHwang, BHSeo, JHCha, HJ
Date Issued
Jan-2014
Publisher
AMER SOC MICROBIOLOGY
Abstract
It is important to rapidly and selectively detect and analyze pathogenic Salmonella enterica subsp. enterica in contaminated food to reduce the morbidity and mortality of Salmonella infection and to guarantee food safety. In the present work, we developed an oligonucleotide microarray containing duplicate specific capture probes based on the carB gene, which encodes the carbamoyl phosphate synthetase large subunit, as a competent biomarker evaluated by genetic analysis to selectively and efficiently detect and discriminate three S. enterica subsp. enterica serotypes: Choleraesuis, Enteritidis, and Typhimurium. Using the developed microarray system, three serotype targets were successfully analyzed in a range as low as 1.6 to 3.1 nM and were specifically discriminated from each other without nonspecific signals. In addition, the constructed microarray did not have cross-reactivity with other common pathogenic bacteria and even enabled the clear discrimination of the target Salmonella serotype from a bacterial mixture. Therefore, these results demonstrated that our novel carB-based oligonucleotide microarray can be used as an effective and specific detection system for S. enterica subsp. enterica serotypes.
URI
http://oasis.postech.ac.kr/handle/2014.oak/9356
ISSN
0099-2240
Article Type
Article
Citation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 80, no. 1, page. 366 - 373, 2014-01
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차형준CHA, HYUNG JOON
Dept. of Chemical Enginrg
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