The tumorigenic, invasive and metastatic potential of epithelial and round subpopulations of the SW480 human colon cancer cell line
SCIE
SCOPUS
- Title
- The tumorigenic, invasive and metastatic potential of epithelial and round subpopulations of the SW480 human colon cancer cell line
- Authors
- YUN, EUN JIN; Yoon, Wan-Hee; Lee, Sang-Kwang; Song, Kyoungsub; Kim, Jong-Seok; Kim, Tae-Dong; Li, Ge; Heo, Jun-Young; Jung, Yeon-Joo; Park, Jong-Il; Kweon, Gi Ryang; Koo, Sun-Hoe; Park, Hae-Duck; Hwang, Byung-Doo; Lim, Kyu
- Date Issued
- 2008-09
- Publisher
- SPANDIDOS PUBL LTD
- Abstract
- It has been reported that the SW480 human colon cancer cell line consists of E-type and R-type cells. The long-term tumorigenic potential, invasive and metastatic properties of these subclones have not been characterized. E-type and R-type cells were subcloned using limiting dilution methods from parental SW480 cells. The cell growth rate was determined by MTT colorimetric assay, and colony forming efficiency was analyzed using Matrigel-coated plates. The activity of matrix metalloproteinase (MMP) and of urokinase plasminogen activator (uPA) was assessed by zymography. Invasive and locomotive ability was analyzed using trans-well chambers. In situ apoptosis detection of these subclones was also performed. In vivo long-term tumorigenicity and nodal metastasis were evaluated using nude mice. E-type cells produced spontaneously regressive tumors in spite of invasion and lymph node metastasis. In contrast, R-type cells revealed progressively growing tumors without invasion or metastasis. E-type cells exhibited increased apoptosis and invasive and motile ability, as well as strong MMP-9 and -2 activity. Although phorbol 12-myristate 13-acetate treatment induced MMP-9 activity in E-type cells, it had no effect on R-type cells. These findings suggest that E-type and R-type cells may have different biological properties in terms of colon cancer progression, regression, invasion and nodal metastasis, and might serve as a useful model for these studies.
- Keywords
- GENETIC INSTABILITY; FACTOR-BETA; GROWTH; MUTATIONS; CARCINOMAS; EXPRESSION; PROTEIN; TUMORS; LUNG
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/40846
- DOI
- 10.3892/mmr_00000026
- ISSN
- 1791-2997
- Article Type
- Article
- Citation
- Molecular Medicine Reports, vol. 1, no. 5, page. 763 - 768, 2008-09
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- There are no files associated with this item.
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