Apicularen A acetate induced cell death via AIF translocation and disrupts the microtubule network by down-regulating tubulin in HM7 human colon cancer cells
SCIE
SCOPUS
- Title
- Apicularen A acetate induced cell death via AIF translocation and disrupts the microtubule network by down-regulating tubulin in HM7 human colon cancer cells
- Authors
- YUN, EUN JIN; Seo, Kang-Sik; Kim, Hoon; Hong, Tae-Hwa; Kim, Jong-Seok; Song, Kyoung-Sub; Park, Ji-Hoon; Jung, Young-Hoon; Park, Jong-Il; Kweon, Gi Ryang; Yoon, Wan-Hee; Lim, Kyu; Hwang, Byung-Doo
- Date Issued
- 2013-05-10
- Publisher
- ELSEVIER
- Abstract
- Apicularen A is a novel antitumor agent and strongly induces death in tumor cells. In this study, we synthesized apicularen A acetate, an acetyl derivative of apicularen A, and investigated its antitumor effect and mechanism in HM7 colon cancer cells. Apicularen A acetate induced apoptotic cell death and caspase-3 activation; however, the pan-caspase inhibitor Z-VAD-fmk could not prevent this cell death. Apicularen A acetate induced the loss of mitochondrial membrane potential and the translocation of apoptosis-inducing factor (AIF) from mitochondria. In addition, apicularen A acetate significantly decreased tubulin mRNA and protein levels and induced disruption of microtubule networks. Taken together, these results indicate that the mechanism of apicularen A acetate involves caspase-independent apoptotic cell death and disruption of microtubule architecture. (C) 2013 Elsevier Inc. All rights reserved.
- Keywords
- INDUCED APOPTOSIS; RAW-264.7 CELLS; INDUCTION; LINE; FAS; CASPASE-3
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/40838
- DOI
- 10.1016/j.bbrc.2013.03.133
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 434, no. 3, page. 634 - 640, 2013-05-10
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