DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, MS | - |
dc.contributor.author | Shin, J | - |
dc.contributor.author | Lee, WT | - |
dc.contributor.author | Lee, HS | - |
dc.contributor.author | Oh, BH | - |
dc.date.accessioned | 2018-01-09T09:01:37Z | - |
dc.date.available | 2018-01-09T09:01:37Z | - |
dc.date.created | 2017-08-03 | - |
dc.date.issued | 2003-07-25 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/40825 | - |
dc.description.abstract | RbsD is the only protein whose biochemical function is unknown among the six gene products of the rbs operon involved in the active transport of ribose. FucU, a paralogue of RbsD conserved from bacteria to human, is also the only protein whose function is unknown among the seven gene products of the L-fucose regulon. Here we report the crystal structures of Bacillus subtilis RbsD, which reveals a novel decameric toroidal assembly of the protein. Nuclear magnetic resonance and other studies on RbsD reveal that the intersubunit cleft of the protein binds specific forms of D-ribose, but it does not have an enzyme activity toward the sugar. Likewise, FucU binds L-fucose but lacks an enzyme activity toward this sugar. We conclude that RbsD and FucU are cytoplasmic sugar-binding proteins, a novel class of proteins whose functional role may lie in helping influx of the sugar substrates. | - |
dc.language | English | - |
dc.publisher | American Society for biochemistry and molecular biology | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.title | Crystal structures of RbsD leading to the identification of cytoplasmic sugar-binding proteins with a novel folding architecture | - |
dc.type | Article | - |
dc.identifier.doi | 10.1074/jbc.M304523200 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.278, no.30, pp.28173 - 28180 | - |
dc.identifier.wosid | 000184242700106 | - |
dc.citation.endPage | 28180 | - |
dc.citation.number | 30 | - |
dc.citation.startPage | 28173 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 278 | - |
dc.contributor.affiliatedAuthor | Kim, MS | - |
dc.identifier.scopusid | 2-s2.0-0041344457 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | COMPLETE GENOME SEQUENCE | - |
dc.subject.keywordPlus | ELECTRON-DENSITY MAPS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | L-FUCOSE | - |
dc.subject.keywordPlus | RIBOSE | - |
dc.subject.keywordPlus | RIBOKINASE | - |
dc.subject.keywordPlus | FORMS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | REGULON | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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