Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
SCIE
SCOPUS
- Title
- Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
- Authors
- Kim, DU; Roh, TY; Lee, J; Noh, JY; Jang, YJ; Hoe, KL; Yoo, HS; Choi, MU
- Date Issued
- 1999-03-25
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLD alpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDa and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD. (C) 1999 Elsevier Science B.V. All rights reserved.
- Keywords
- phospholipase D; cDNA sequence; expression; (Brassica oleracea var. capitata); ADP-RIBOSYLATION FACTOR; D DEFINES; PLD-BETA; PURIFICATION; PHOSPHATIDYLCHOLINE; FAMILY; ALPHA; HYDROLYSIS; FORMS; GAMMA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28977
- DOI
- 10.1016/S1388-1981(99)00020-7
- ISSN
- 1388-1981
- Article Type
- Article
- Citation
- BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, vol. 1437, no. 3, page. 409 - 414, 1999-03-25
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.